Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response
| العنوان: | Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response |
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| المؤلفون: | Renzo Boldorini, Martina Ramella, Maria Talmon, Luigia Grazia Fresu, Luca Fusaro, Carla Porta, Francesco Casella, Giulia Bertozzi, Marcello Manfredi, Francesca Boccafoschi, Emilio Marengo, Marta Calvo Catoria |
| المصدر: | International Journal of Molecular Sciences, Vol 20, Iss 2, p 287 (2019) International Journal of Molecular Sciences Volume 20 Issue 2 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, Cell Culture Techniques, Stimulation, 030204 cardiovascular system & hematology, calcification, lcsh:Chemistry, Bioreactors, 0302 clinical medicine, oxidative stress, Gene Regulatory Networks, lcsh:QH301-705.5, Spectroscopy, cyclic stretch, Chemistry, General Medicine, Abdominal aortic aneurysm, Computer Science Applications, Endothelial stem cell, Matrix Metalloproteinase 9, cardiovascular system, Tumor necrosis factor alpha, medicine.symptom, Extracellular Matrix Degradation, medicine.medical_specialty, Inflammation, Models, Biological, Article, Catalysis, Cell Line, Inorganic Chemistry, 03 medical and health sciences, abdominal aortic aneurysm, medicine.artery, medicine, Humans, Physical and Theoretical Chemistry, Molecular Biology, Aorta, Tumor Necrosis Factor-alpha, Organic Chemistry, Endothelial Cells, medicine.disease, cardiovascular diseases, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, inflammation, Stress, Mechanical, Aortic Aneurysm, Abdominal, Calcification |
| الوصف: | Abdominal aortic aneurysm (AAA) is a focal dilatation of the aorta, caused by both genetic and environmental factors. Although vascular endothelium plays a key role in AAA progression, the biological mechanisms underlying the mechanical stress involvement are only partially understood. In this study, we developed an in vitro model to characterize the role of mechanical stress as a potential trigger of endothelial deregulation in terms of inflammatory response bridging between endothelial cells (ECs), inflammatory cells, and matrix remodeling. In AAA patients, data revealed different degrees of calcification, inversely correlated with wall stretching and also with inflammation and extracellular matrix degradation. In order to study the role of mechanical stimulation, endothelial cell line (EA.hy926) has been cultured in healthy (10% strain) and pathological (5% strain) dynamic conditions using a bioreactor. In presence of tumor necrosis factor alpha (TNF-&alpha ), high levels of matrix metalloproteinase-9 (MMP-9) expression and inflammation are obtained, while mechanical stimulation significantly counteracts the TNF-&alpha effects. Moreover, physiological deformation also plays a significant role in the control of the oxidative stress. Overall our findings indicate that, due to wall calcification, in AAA there is a significant change in terms of decreased wall stretching. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9ede458fe429fa1b4f1c8f60cabf067 https://doi.org/10.3390/ijms20020287 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....c9ede458fe429fa1b4f1c8f60cabf067 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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