Correction to : Mapping of leptin and its syntenic genes to chicken chromosome 1p
| العنوان: | Correction to : Mapping of leptin and its syntenic genes to chicken chromosome 1p |
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| المؤلفون: | Sara Yosefi, Susanne Bornelöv, Miriam Friedman-Einat, Frédérique Pitel, Larry A. Cogburn, Eyal Seroussi, M. Morisson, Leif Andersson, Shoval Miyara, David W. Burt, Sophie Leroux |
| المصدر: | BMC Genetics, Vol 18, Iss 1, Pp 1-1 (2017) BMC Genetics |
| بيانات النشر: | Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Genetics, lcsh:QH426-470, Leptin, Chicken leptin, Public Health, Global Health, Social Medicine and Epidemiology, chicken RBM28, Syntenic clusters, Biology, 03 medical and health sciences, lcsh:Genetics, Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi, 030104 developmental biology, Chromosome (genetic algorithm), GC-rich, Genetik, Gene, Author name, Genetics (clinical), Research Article, chicken chromosome 1, Synteny |
| الوصف: | Background Misidentification of the chicken leptin gene has hampered research of leptin signaling in this species for almost two decades. Recently, the genuine leptin gene with a GC-rich (~70%) repetitive-sequence content was identified in the chicken genome but without indicating its genomic position. This suggests that such GC-rich sequences are difficult to sequence and therefore substantial regions are missing from the current chicken genome assembly. Results A radiation hybrid panel of chicken-hamster Wg3hCl2 cells was used to map the genome location of the chicken leptin gene. Contrary to our expectations, based on comparative genome mapping and sequence characteristics, the chicken leptin was not located on a microchromosome, which are known to contain GC-rich and repetitive regions, but at the distal tip of the largest chromosome (1p). Following conserved synteny with other vertebrates, we also mapped five additional genes to this genomic region (ARF5, SND1, LRRC4, RBM28, and FLNC), bridging the genomic gap in the current Galgal5 build for this chromosome region. All of the short scaffolds containing these genes were found to consist of GC-rich (54 to 65%) sequences comparing to the average GC-content of 40% on chromosome 1. In this syntenic group, the RNA-binding protein 28 (RBM28) was in closest proximity to leptin. We deduced the full-length of the RBM28 cDNA sequence and profiled its expression patterns detecting a negative correlation (R = − 0.7) between the expression of leptin and of RBM28 across tissues that expressed at least one of the genes above the average level. This observation suggested a local regulatory interaction between these genes. In adipose tissues, we observed a significant increase in RBM28 mRNA expression in breeds with lean phenotypes. Conclusion Mapping chicken leptin together with a cluster of five syntenic genes provided the final proof for its identification as the true chicken ortholog. The high GC-content observed for the chicken leptin syntenic group suggests that other similar clusters of genes in GC-rich genomic regions are missing from the current genome assembly (Galgal5), which should be resolved in future assemblies of the chicken genome. Electronic supplementary material The online version of this article (doi:10.1186/s12863-017-0543-1) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca02f9c87e0a5387fc872b41743f124f http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-347716 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ca02f9c87e0a5387fc872b41743f124f |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |