α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway
| العنوان: | α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway |
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| المؤلفون: | Jun-Qiang Chen, Ting Chen, Ye Wang, Kaitian Zheng, Jiancheng Wang, Yeyang Chen, Bo-Pei Li, Kun Zhao, Zhen Wang, Junfu Wang, Zhu Yu, Siwen Zhang, Weijia Huang, Congjun Wang, Zhao Fu |
| المصدر: | Bioengineered, Vol 12, Iss 1, Pp 5688-5704 (2021) Bioengineered article-version (VoR) Version of Record |
| بيانات النشر: | Taylor & Francis Group, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, akt/gsk3β/β-catenin, Epithelial-Mesenchymal Transition, Carcinogenesis, Bioengineering, medicine.disease_cause, Applied Microbiology and Biotechnology, Stomach Neoplasms, Cell Line, Tumor, medicine, Humans, Actinin, Epithelial–mesenchymal transition, Cell adhesion, Protein kinase B, beta Catenin, Aged, α-actinin1, Glycogen Synthase Kinase 3 beta, Cell growth, Chemistry, gastric cancer, apoptosis, Cancer, General Medicine, Middle Aged, medicine.disease, Catenin, Cancer cell, Cancer research, Female, epithelial-to-mesenchymal transition, Proto-Oncogene Proteins c-akt, TP248.13-248.65, Signal Transduction, Research Article, Research Paper, Biotechnology |
| الوصف: | α-Actinin1 (ACTN1), an actin cross-linking protein, is implicated in cytokinesis, cell adhesion, and cell migration. In addition, it is involved in the tumorigenesis and development of certain cancers, such as breast cancer. We explored the function of ACTN1 in gastric cancer (GC), which has largely remained unclear. High-throughput sequencing and public microarray datasets from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) revealed the upregulation of ACTN1 in gastric cancer with a poor prognosis. These results were further verified by western blotting (WB), Real-Time Quantitative polymerase chain reaction (RT-qPCR), and immunohistochemistry. We constructed loss and gain of function gastric cancer cells, which revealed the effect of ACTN1 over-expression on promoting GC cell proliferation, invasion, migration, and inhibited apoptosis. Mechanistic studies revealed that ACTN1 regulates the epithelial-mesenchymal transition (EMT) and tumorigenesis of gastric cancer via the AKT/GSK3β/β-catenin pathway, confirmed by the inhibitor of AKT MK2206. Altogether, these results demonstrated that ACTN1 could be a promising candidate for gastric cancer treatment. |
| اللغة: | English |
| تدمد: | 2165-5987 2165-5979 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca76da9580521441cd05f05e08a09059 https://doaj.org/article/9cb963a70f544d4fbcc1cd4262213e24 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ca76da9580521441cd05f05e08a09059 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 21655987 21655979 |
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