Effect of intermittent hypoxia on the cardiac HIF-1/VEGF pathway in experimental type 1 diabetes mellitus
| العنوان: | Effect of intermittent hypoxia on the cardiac HIF-1/VEGF pathway in experimental type 1 diabetes mellitus |
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| المؤلفون: | Bizden Sabuncuoğlu, Firat Akat, Derya Güzel, Ali Dursun, Demet Tekin, Metin Bastug, Ferda Topal Çelikkan, Ayhan Tanyeli, Hakan Fiçicilar |
| المساهمون: | Guzel, D, Dursun, AD, Ficicilar, H, Tekin, D, Tanyeli, A, Akat, F, Celikkan, FT, Sabuncuoglu, B, Bastug, M, Sakarya Üniversitesi/Tıp Fakültesi/Temel Tıp Bilimleri Bölümü, Güzel, Derya |
| المصدر: | Anatolian Journal of Cardiology |
| بيانات النشر: | Kare Publishing, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Cardiac function curve, Male, Vascular Endothelial Growth Factor A, medicine.medical_specialty, Diabetic Cardiomyopathies, intermittent hypoxia, chemistry.chemical_compound, angiogenesis, Diabetic cardiomyopathy, Internal medicine, Diabetes mellitus, medicine, diabetic cardiomyopathy, Animals, Rats, Wistar, Hypoxia, Original Investigation, business.industry, Weight change, HIF-1, Intermittent hypoxia, Hypoxia (medical), medicine.disease, Streptozotocin, VEGF, Surgery, Rats, Vascular endothelial growth factor, Endocrinology, Diabetes Mellitus, Type 1, chemistry, Cardiovascular System & Cardiology, sense organs, Hypoxia-Inducible Factor 1, medicine.symptom, Cardiology and Cardiovascular Medicine, business, medicine.drug |
| الوصف: | Objective High altitude and hypoxic preconditioning have cardioprotective effects by increasing coronary vascularity, reducing post-ischemic injury, and improving cardiac function. Our purpose was to examine if intermittent hypoxia treatment has any restoring effects related to the possible role of the HIF-1/VEGF pathway on diabetic cardiomyopathy. Methods Wistar Albino male rats (n=34) were divided into four groups: control (C), intermittent hypoxia (IH), diabetes mellitus (DM), and diabetes mellitus plus intermittent hypoxia (DM+IH). Following a streptozotocin (STZ) injection (50 mg/kg, i.p.), blood glucose levels of 250 mg/dL and above were considered as DM. IH and DM+IH groups were exposed to hypoxia 6 h/day for 42 days at a pressure corresponding to 3000 m altitude. Twenty-four hours after the IH protocol, hearts were excised. Hematoxylin and eosin-stained apical parts of the left ventricles were evaluated. Hypoxia inducible factor-1 (HIF-1), vascular endothelial growth factor 164 (VEGF164), and VEGF188 polymerase chain reaction products were run in agarose gel electrophoresis. Band density analysis of UV camera images was performed using Image J. The data were compared by one-way ANOVA, repeated measures two-way ANOVA, and the Kruskal-Wallis test. Results The percent weight change was lower in the DM group than in the controls (p=0.004). The tissue injury was the highest in the DM group and the least in the IH group. Diabetes decreased, whereas the IH treatment increased the vascularity. A decrease was observed in the VEGF188 mRNA levels in the DM+IH group compared with the C group, but there were no difference in HIF-1α and VEGF164 mRNA levels between the groups. Conclusion The IH treatment restored the diabetic effects on the heart by reducing tissue injury and increasing the capillarity without transcriptional changes in HIF-1/VEGF correspondingly. |
| اللغة: | English |
| تدمد: | 2149-2271 2149-2263 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cac8e77799cc1c7b5a5ef16d85ea02fc http://europepmc.org/articles/PMC5336740 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....cac8e77799cc1c7b5a5ef16d85ea02fc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21492271 21492263 |
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