PGC-1α attenuates the oxidative stress-induced impaired osteogenesis and angiogenesis regulation effects of mesenchymal stem cells in the presence of diabetic serum
| العنوان: | PGC-1α attenuates the oxidative stress-induced impaired osteogenesis and angiogenesis regulation effects of mesenchymal stem cells in the presence of diabetic serum |
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| المؤلفون: | Shikun Wang, Zongxin Shi, Zishun Gong, Jiechao Deng |
| المصدر: | Biochemistry and Biophysics Reports, Vol 27, Iss, Pp 101070-(2021) Biochemistry and Biophysics Reports |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Angiogenesis, Chemistry, QH301-705.5, Mesenchymal stem cell, Biophysics, PGC-1α, Oxidative phosphorylation, QD415-436, medicine.disease_cause, Biochemistry, Cell biology, Bone remodeling, Mitochondrial permeability transition pore, Mitochondrial biogenesis, Oxidative stress, Osteogenesis, medicine, Biology (General), Receptor, Research Article |
| الوصف: | Oxidative stress is believed to induce dysfunction of the bone remodeling process and be associated with progressive loss of bone mass. The peroxisome proliferator-activated receptor gamma coactivator 1α (PGC-1α) is a master controller during mitochondrial biogenesis and the antioxidant response. We postulated that PGC-1α could function as a cyto-protective effector in mesenchymal stem cells (MSCs) under oxidative stress conditions. In this study, diabetic serum was firstly used to treat MSCs to induce oxidative damage. The anti-oxidative protective effects of PGC-1α overexpression on MSCs, as well as MSCs’ osteogenesis and angiogenic regulation effects were investigated in vitro. Results showed that diabetic conditions induced significantly increase of intracellular oxidative damage and mitochondrial permeability transition pore (mPTP) opening activity, decrease of cellular viability, and osteogenic differentiation and pro-angiogenic regulation effects of MSCs. However, the diabetic conditions induced oxidative impair on MSCs were significantly alleviated via PGC-1α overexpression under diabetic conditions. Taken together, this study indicates the anti-oxidative treatment potential of PGC-1α regulation as a promising strategy to promote coupling pro-osteogenesis and pro-angiogenesis effects of MSCs. Highlights • Diabetic induced intracellular oxidative damage, and decreased osteogenic and pro-angiogenic regulation effects of MSCs. • PGC-1α overexpression protects the MSCs from diabetic conditions induced oxidative impair. • PGC-1α overexpression contributed to enhanced osteogenic and pro-angiogenic regulation effects of MSCs under diabetic conditions. |
| اللغة: | English |
| تدمد: | 2405-5808 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb33bf7cad928afcafec498e2e9e5e45 http://www.sciencedirect.com/science/article/pii/S2405580821001643 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....cb33bf7cad928afcafec498e2e9e5e45 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24055808 |
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