Mechanism by which the combination of SjCL3 and SjGAPDH protects against Schistosoma japonicum infection
| العنوان: | Mechanism by which the combination of SjCL3 and SjGAPDH protects against Schistosoma japonicum infection |
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| المؤلفون: | Wenling Huang, Hui-Fen Dong, Kaijie Li, Zhenping Ming, Qin-Ping Zhao, Mengjie Gu, Wen-Jun Cheng, Shuhong Wang |
| المصدر: | Parasitology Research. 120:173-185 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | medicine.medical_treatment, 030231 tropical medicine, Schistosomiasis, Biology, T-Lymphocytes, Regulatory, Schistosoma japonicum, 030308 mycology & parasitology, Microbiology, Mice, 03 medical and health sciences, 0302 clinical medicine, Immune system, parasitic diseases, medicine, Animals, Cathepsin, Mice, Inbred BALB C, Vaccines, 0303 health sciences, Schistosoma Japonicum Infection, General Veterinary, Vaccination, Glyceraldehyde-3-Phosphate Dehydrogenases, Helminth Proteins, General Medicine, medicine.disease, biology.organism_classification, Cathepsins, Infectious Diseases, Immunization, Schistosomiasis japonica, Insect Science, Female, Parasitology, Adjuvant |
| الوصف: | A vaccine is an important method to control schistosomiasis. Molecules related to lung-stage schistosomulum are considered potential vaccine candidates. We previously showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and cathepsin L3 (CL3) displayed differential expression in the lung-stage schistosomula of Schistosoma japonicum cocultured with host cells. In the present study, we prepared the two proteins and detected the protective effects of SjGAPDH by immunizing mice with this protein alone and in combination with SjCL3 with or without Freund's adjuvant. Then, we investigated the possible mechanisms underlying S. japonicum infection. The results showed that vaccination of adjuvanted SjGAPDH decreased the worm burden (37.8%) and egg load (38.1%), and the combination of adjuvanted SjGAPDH and SjCL3 further decreased the worm burden (65.6%) and egg load (70.9%) during Schistosoma japonicum infection. However, the immunization of a combination of adjuvant-free SjGAPDH and SjCL3 displayed a lower protective effect (15%) than those of the adjuvanted SjCL3, the adjuvanted SjGAPDH, and a combination of adjuvanted SjGAPDH and SjCL3. Flow cytometric results showed that the frequency of regulatory T cells (Tregs) was lower (P0.05) in the group with adjuvanted SjGAPDH and SjCL3 (2.61%) than the remaining groups. The enzyme-linked immunosorbent assay (ELISA) results indicated that except for the uninfected and infected control groups, the remaining groups displayed a Th1-type shift in immune responses. These results showed the immunization of SjGAPDH resulted in partial protection (approximately 38%); inoculation with a combination of SjCL3 and SjGAPDH in Freund's adjuvant resulted in a high immunoprotective effect (65%) against Schistosoma japonicum infection in mice, which was possibly caused by the reduced percentage of Tregs and a Th1-type shift in immune responses; and SjCL3 has no adjuvant-like effect, dissimilar to SmCL3. |
| تدمد: | 1432-1955 0932-0113 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb4904aee62b6db7de0e5bffbae93b64 https://doi.org/10.1007/s00436-020-06916-9 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....cb4904aee62b6db7de0e5bffbae93b64 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14321955 09320113 |
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