التفاصيل البيبلوغرافية
| العنوان: |
Plasmid‐Based Donor Templates for Nonviral CRISPR/Cas9‐Mediated Gene Knock‐In in Human T Cells |
| المؤلفون: |
Kate, Senger, Ilseyar, Akhmetzyanova, Benjamin, Haley, Sascha, Rutz, Soyoung A, Oh |
| المصدر: |
Current Protocols. 2 |
| بيانات النشر: |
Wiley, 2022. |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
General Immunology and Microbiology, General Neuroscience, Receptors, Antigen, T-Cell, Health Informatics, DNA, CD8-Positive T-Lymphocytes, General Biochemistry, Genetics and Molecular Biology, Medical Laboratory Technology, CD28 Antigens, Ribonucleoproteins, Humans, Gene Knock-In Techniques, CRISPR-Cas Systems, General Pharmacology, Toxicology and Pharmaceutics, Plasmids |
| الوصف: |
Effective and precise gene editing of T lymphocytes is critical for advancing the understanding of T cell biology and the development of next-generation cellular therapies. Although methods for effective CRISPR/Cas9-mediated gene knock-out in primary human T cells have been developed, complementary techniques for nonviral gene knock-in can be cumbersome and inefficient. Here, we report a simple and efficient method for nonviral CRISPR/Cas9-based gene knock-in utilizing plasmid-based donor DNA templates. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Purification of human CD4 |
| تدمد: |
2691-1299 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbfcd66d09a625e10782ed53ece78e39
https://doi.org/10.1002/cpz1.538 |
| حقوق: |
CLOSED |
| رقم الأكسشن: |
edsair.doi.dedup.....cbfcd66d09a625e10782ed53ece78e39 |
| قاعدة البيانات: |
OpenAIRE |
