Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum
| العنوان: | Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum |
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| المؤلفون: | F. Boué, Paul Anderson, Sylvie Hénault, Gareth A. Williams, Céline Richomme, Christoph Wanke, Sonya Gowtage, Dipesh Davé, Mark A. Chambers, Jacques Barrat, Elodie Monchâtre-Leroy, Sandrine Lesellier, John Spiropoulos, Maria-Laura Boschiroli, S. Palmer, Jean-Marc Boucher, A. Núñez, Ana Godinho, Waldo L. García-Jiménez, Allan K. Nadian, Francisco J. Salguero, Lorraine Michelet, Krystel de Cruz |
| المساهمون: | Animal and Plant Health Agency (APHA), Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, High Tech Campus Eindhoven, Public Health England [London], University of Surrey (UNIS) |
| المصدر: | BMC Veterinary Research BMC Veterinary Research, BioMed Central, 2019, 15 (1), ⟨10.1186/s12917-019-2166-4⟩ BMC Veterinary Research, Vol 15, Iss 1, Pp 1-15 (2019) |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Lymphatic drainage, Badger, [SDV]Life Sciences [q-bio], Administration, Oral, 0403 veterinary science, Feces, Mesenteric lymph nodes, BCG, Capsule, 0303 health sciences, Mycobacterium bovis, lcsh:Veterinary medicine, biology, Vaccination, 04 agricultural and veterinary sciences, General Medicine, 3. Good health, medicine.anatomical_structure, Lymphatic system, BCG Vaccine, Female, Lymph, Research Article, 040301 veterinary sciences, Ileum, Meles, complex mixtures, Mucosa, Interferon-gamma, 03 medical and health sciences, biology.animal, Mustelidae, medicine, Animals, Tuberculosis, 030304 developmental biology, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, General Veterinary, business.industry, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Tonsil, Delayed-Action Preparations, Immunology, lcsh:SF600-1100, Lymph Nodes, business, Vaccine |
| الوصف: | International audience; BackgroundOral vaccination with Mycobacterium bovis Bacille of Calmette and Guerin (BCG) has provided protection against M. bovis to badgers both experimentally and in the field. There is also evidence suggesting that the persistence of live BCG within the host is important for maintaining protection against TB. Here we investigated the capacity of badger inductive mucosal sites to absorb and maintain live BCG. The targeted mucosae were the oropharyngeal cavity (tonsils and sublingual area) and the small intestine (ileum).ResultsWe showed that significant quantities of live BCG persisted within badger in tissues of vaccinated badgers for at least 8 weeks following oral vaccination with only very mild pathological features and induced the circulation of IFNγ-producing mononuclear cells. The uptake of live BCG by tonsils and drainage to retro-pharyngeal lymph nodes was repeatable in the animal group vaccinated by oropharyngeal instillation whereas those vaccinated directly in the ileum displayed a lower frequency of BCG detection in the enteric wall or draining mesenteric lymph nodes. No faecal excretion of live BCG was observed, including when BCG was delivered directly in the ileum.ConclusionsThe apparent local loss of BCG viability suggests an unfavorable gastro-enteric environment for BCG in badgers, which should be taken in consideration when developing an oral vaccine for use in this species. |
| اللغة: | English |
| تدمد: | 1746-6148 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc224f1194cce00c32b4fbe975af6623 https://hal-anses.archives-ouvertes.fr/anses-03298045/file/s12917-019-2166-4.pdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....cc224f1194cce00c32b4fbe975af6623 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 17466148 |
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