Inhibitory effects of rosiglitazone on paraquat-induced acute lung injury in rats
| العنوان: | Inhibitory effects of rosiglitazone on paraquat-induced acute lung injury in rats |
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| المؤلفون: | Wei-jian Hou, Zhenning Liu, Shu-ling Bai, Hongyu Zhao, Min Zhao, Qiang Zheng |
| المصدر: | Acta Pharmacologica Sinica. 34:1317-1324 |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Paraquat, Agonist, Time Factors, NF-E2-Related Factor 2, medicine.drug_class, Acute Lung Injury, Interleukin-1beta, Pulmonary Edema, Lung injury, Pharmacology, Rats, Sprague-Dawley, Rosiglitazone, chemistry.chemical_compound, Malondialdehyde, medicine, Animals, Pharmacology (medical), Dose-Response Relationship, Drug, Herbicides, Superoxide Dismutase, Tumor Necrosis Factor-alpha, business.industry, NF-kappa B, General Medicine, Pulmonary edema, medicine.disease, Rats, PPAR gamma, IκBα, Dose–response relationship, chemistry, Anesthesia, Original Article, Thiazolidinediones, business, medicine.drug |
| الوصف: | To investigate the effects of the PPAR-γ agonist rosiglitazone on acute lung injury induced by the herbicide paraquat (PQ) and the underlying mechanisms of action. Male Sprague-Dawley rats were injected with PQ (20 mg/kg, ip). Rosiglitazone (3 or 10 mg/kg, ip) was administered 1 h before PQ exposure. Peripheral blood was collected at 4, 8, 24 and 72 h after PQ exposure for measuring the levels of MDA, TNF-α and IL-1β, and the SOD activity. Lung tissues were collected at 72 h after PQ exposure to determine the wet-to-dry (W/D) ratios and lung injury scores, as well as the protein levels of NF-κBp65, PPAR-γ, Nrf2, IκBα and pIκBα. At 72 h after PQ exposure, the untreated rats showed a 100% cumulative mortality, whereas no death was observed in rosiglitazone-pretreated rats. Moreover, rosiglitazone pretreatment dose-dependently attenuated PQ-induced lung edema and lung histopathological changes. The pretreatment significantly reduced the levels of TNF-α, IL-1β and MDA, increased SOD activity in the peripheral blood of PQ-treated rats. The pretreatment also efficiently activated PPAR-γ, induced Nrf2 expression and inhibited NF-κB activation in the lung tissues of PQ-treated rats. Furthermore, the pretreatment dose-dependently inhibited IκB-α degradation and phosphorylation, thus inhibiting NF-κB activation. Pretreatment with rosiglitazone protects rats against PQ-induced acute lung injury by activating PPAR-γ, inducing Nrf2 expression and inhibiting NF-κB activation. |
| تدمد: | 1745-7254 1671-4083 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc55954045b48abe1fee94483be143a1 https://doi.org/10.1038/aps.2013.65 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....cc55954045b48abe1fee94483be143a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17457254 16714083 |
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