Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles
| العنوان: | Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles |
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| المؤلفون: | Charles P. Lai, Eduardo Reátegui, Fred H. Hochberg, Aimal H. Khankhel, Bob S. Carter, Shannon L. Stott, Mahnaz Zeinali, Xandra O. Breakefield, Kristan E. van der Vos, Nadia A. Atai, Vishal Thapar, Leonora Balaj, Frederick P. Floyd, Berent Aldikacti, Lecia V. Sequist, Mehmet Toner, David T. Ting, Brian V. Nahed |
| المصدر: | Nature Communications Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018) |
| بيانات النشر: | Nature Publishing Group UK, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Science, Microfluidics, Mutant, General Physics and Astronomy, Extracellular vesicles, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, chemistry.chemical_compound, Extracellular Vesicles, Cell Line, Tumor, Humans, lcsh:Science, Gene, Messenger RNA, Multidisciplinary, Brain Neoplasms, RNA, Biological Transport, General Chemistry, 3. Good health, Cell biology, ErbB Receptors, 030104 developmental biology, chemistry, Cell culture, lcsh:Q, Glioblastoma, DNA |
| الوصف: | Extracellular vesicles (EVs) carry RNA, DNA, proteins, and lipids. Specifically, tumor-derived EVs have the potential to be utilized as disease-specific biomarkers. However, a lack of methods to isolate tumor-specific EVs has limited their use in clinical settings. Here we report a sensitive analytical microfluidic platform (EVHB-Chip) that enables tumor-specific EV-RNA isolation within 3 h. Using the EVHB-Chip, we achieve 94% tumor-EV specificity, a limit of detection of 100 EVs per μL, and a 10-fold increase in tumor RNA enrichment in comparison to other methods. Our approach allows for the subsequent release of captured tumor EVs, enabling downstream characterization and functional studies. Processing serum and plasma samples from glioblastoma multiforme (GBM) patients, we can detect the mutant EGFRvIII mRNA. Moreover, using next-generation RNA sequencing, we identify genes specific to GBM as well as transcripts that are hallmarks for the four genetic subtypes of the disease. Extracellular vesicles can carry many different types of biological cargo and have been investigated as a biomarker for cancer diagnosis. Here the authors develop a microfluidic platform for rapid and sensitive isolation of tumor-specific extracellular vesicles. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc7771e4d608e23327db5d7b7ebd2021 http://europepmc.org/articles/PMC5766611 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....cc7771e4d608e23327db5d7b7ebd2021 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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