Paeoniflorin improves regional cerebral blood flow and suppresses inflammatory factors in the hippocampus of rats with vascular dementia
العنوان: | Paeoniflorin improves regional cerebral blood flow and suppresses inflammatory factors in the hippocampus of rats with vascular dementia |
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المؤلفون: | Li-gong Zhang, Ying Zhang, Meng-yuan Zhang, Cuige Shi, Lijun Wang, Qing-qing Shen, Shucheng Zhang, Haofeng Wang |
المصدر: | Chinese Journal of Integrative Medicine. 23:696-702 |
بيانات النشر: | Springer Science and Business Media LLC, 2015. |
سنة النشر: | 2015 |
مصطلحات موضوعية: | Male, 0301 basic medicine, Down-Regulation, Nitric Oxide Synthase Type II, Hippocampus, Traditional Chinese medicine, Pharmacology, Neuroprotection, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Paeoniae Radix, Glucosides, Animals, Medicine, Cognitive Dysfunction, Pharmacology (medical), Inflammatory factors, RNA, Messenger, Maze Learning, Vascular dementia, Memory Disorders, business.industry, Dementia, Vascular, Transcription Factor RelA, General Medicine, Paeoniflorin, medicine.disease, 030104 developmental biology, Complementary and alternative medicine, Cerebral blood flow, chemistry, Cyclooxygenase 2, Cerebrovascular Circulation, Monoterpenes, Inflammation Mediators, business, 030217 neurology & neurosurgery |
الوصف: | To explore the delayed neuroprotection induced by paeoniflorin (PF), the principal component of Paeoniae radix prescribed in Chinese medicine, and its underlying mechanisms in rats subjected to vascular dementia (VD).A rat model of VD was induced by bilateral common carotid arteries occlusion (BCCAO). Low-dose or high-dose PF (20 or 40 mg/kg once per day) was administrated for 28 days after VD. The behavioral analysis of rat was measured by water morris. Regional cerebral blood volume (rCBV), regional cerebral blood flflow (rCBF) and mean transit time (MTT) were measured in the bilateral hippocampus by perfusion-weighted imaging (PWI). The levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) were measured by commercially available enzyme-linked immunosorbent assay kits. Protein levels were evaluated by western blot analysis. mRNA levels were evaluated by real time-polymerase chain reaction. Western blotting was used to estimate p65 translocation.The behavioral analysis showed that PF could decrease the escape latency time (P0.05), and increase the residence time of the original platform quadrant and the across platform frequency in water maze in VD rats (P0.05). Likewise, PF remarkably promoted the rCBV (P0.05), rCBF and decreased per minute MTT (P0.05) in hippocampus of VD rats. Furthermore, PF decreased the release of IL-1β, IL-6 and TNF-α as well as inhibited the mRNA expression of IL-1β, IL-6 and TNF-α in the hippocampus of VD rats (P0.05 or P0.01). PF also could decrease the protein expressions of inducible nitric oxide synthase and cyclooxygenase-2 in the hippocampus of VD rats (P0.05 or P0.01). In addition, PF signifificantly inhibited the nuclear factor κB (NF-κB) pathway in the hippocampus of VD rats.PF signifificantly attenuates cognitive impairment, improves hippocampus perfusion and inhibits inflflammatory response in VD rats. In addition, the anti-inflflammatory effects of PF might be due to inhibiting the NF-κB pathway. PF may be a potential clinical application in improving VD. |
تدمد: | 1993-0402 1672-0415 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc8608af83976bbcd1c24a2e5354e697 https://doi.org/10.1007/s11655-015-2124-3 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....cc8608af83976bbcd1c24a2e5354e697 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 19930402 16720415 |
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