Turn-off colorimetric sensor for sequence-specific recognition of single-stranded DNA based upon Y-shaped DNA structure
| العنوان: | Turn-off colorimetric sensor for sequence-specific recognition of single-stranded DNA based upon Y-shaped DNA structure |
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| المؤلفون: | Fan He, Mingqin Zhao, Hong Zhang, Xintong Li, Liansheng Ling |
| المصدر: | Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Deoxyribozyme, Oligonucleotides, DNA, Single-Stranded, lcsh:Medicine, 02 engineering and technology, 010402 general chemistry, G-quadruplex, 01 natural sciences, Polymorphism, Single Nucleotide, Sensitivity and Specificity, Article, Absorbance, chemistry.chemical_compound, Limit of Detection, Benzothiazoles, lcsh:Science, Detection limit, Multidisciplinary, biology, Base Sequence, Oligonucleotide, lcsh:R, DNA, Catalytic, Hydrogen Peroxide, 021001 nanoscience & nanotechnology, 0104 chemical sciences, G-Quadruplexes, chemistry, Molecular Probes, biology.protein, Biophysics, Feasibility Studies, Hemin, Colorimetry, Indicators and Reagents, Spectrophotometry, Ultraviolet, lcsh:Q, Sulfonic Acids, 0210 nano-technology, Oxidation-Reduction, DNA, Peroxidase |
| الوصف: | A novel turn-off colorimetric sensor for sequence-specific recognition of single-stranded DNA (ssDNA) was established by combining Y-shaped DNA duplex and G-quadruplex-hemin DNAzyme. A G-rich single-stranded DNA (Oligo-1) displays peroxidase mimicking catalytic activity due to the specific binding with hemin in the presence of K+, which was able to catalyze the oxidation of colorless 2,2′-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS2−) by H2O2 to generate green ABTS•− radical for colorimetric assay. Oligonucleotide 2 (Oligo-2) was partly complementary with Oligo-1 and the target DNA. Upon addition of target DNA, Oligo-1, Oligo-2 and target DNA can hybridize with each other to form Y-shaped DNA duplex. The DNAzyme sequence of Oligo-1 was partly caged into Y-shaped DNA duplex, resulting in the inactivation of the DNAzyme and a sharp decrease of the absorbance of the oxidation product of ABTS2−. Under the optimum condition, the absorbance decreased linearly with the concentration of target DNA over the range of 1.0–250 nM and the detection limit was 0.95 nM (3σ/slope) Moreover, satisfied result was obtained for the discrimination of single-base or two-base mismatched DNA. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc950e36163fe16527b33660f9fb97f9 http://link.springer.com/article/10.1038/s41598-018-30529-z |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....cc950e36163fe16527b33660f9fb97f9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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