Inflammation and insulin resistance exert dual effects on adipose tissue tumor protein 53 expression
| العنوان: | Inflammation and insulin resistance exert dual effects on adipose tissue tumor protein 53 expression |
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| المؤلفون: | F Ortega, Gertrude Mingrone, Gema Frühbeck, Elodie Luche, Francisco J. Tinahones, Dolores Mayas, José Manuel Fernández-Real, José Ignacio Rodríguez-Hermosa, Wifredo Ricart, J M Moreno-Navarrete, Rémy Burcelin, Matteo Serino |
| المصدر: | International Journal of Obesity. 38:737-745 |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Adipose tissue macrophages, Bariatric Surgery, Gene Expression, Medicine (miscellaneous), Adipose tissue, Enzyme-Linked Immunosorbent Assay, Inflammation, White adipose tissue, Diet, High-Fat, Rosiglitazone, Mice, Insulin resistance, Internal medicine, Gene expression, Adipocytes, medicine, Animals, Humans, Obesity, Mice, Knockout, Analysis of Variance, Adipogenesis, Nutrition and Dietetics, Chemistry, Genes, p53, medicine.disease, Metformin, Endocrinology, Adipose Tissue, Female, Thiazolidinediones, Insulin Resistance, medicine.symptom, Omentum, medicine.drug |
| الوصف: | The purpose of this study was to investigate the expression of human adipose tissue protein 53 (p53) in subjects who varied widely in terms of obesity and insulin resistance. We also analyzed different in vivo and in vitro models to try to comprehend the associations found in humans.p53 was analyzed in human adipose and isolated adipocytes, in high fat-fed and GLP-1R KO mice, during in vitro adipogenesis, and in adipocytes after high glucose, rosiglitazone and inflammatory conditions. The effects of surgery-induced weight loss and ex vivo metformin were also evaluated.Omental (OM) p53 gene expression (+27%, P=0.001) and protein (+11%, P=0.04) were increased in obese subjects and high fat diet-induced obese mice (+86%, P=0.018). Although the obesity-associated inflammatory milieu was associated with increased OM p53, this was negatively related to insulin resistance and glycated hemoglobin, and positively with biomarkers for insulin sensitivity. Multiple linear regression analyses revealed that glycated hemoglobin (P0.0001) and body mass index (P=0.048) contributed independently to explain 13.7% (P0.0001) of the OM p53 variance. Accordingly, the improvement of insulin sensitivity with surgery-induced weight loss (+51%, P=0.01) and metformin (+42%, P=0.02) led to increased adipose p53. While the glucose-intolerant GLP-1R KO mice showed decreased mesenteric p53 (-45.4%, P=0.017), high glucose led to decreased p53 in pre-adipocytes (-27%, P0.0001). Inflammatory treatments led to increased p53 (+35%, P0.0001), while Rs downregulated this expression (-40%, P=0.005) in mature adipocytes.Inflammation and insulin resistance exert dual effects on adipose p53, which seems to be the final result of these opposing forces. |
| تدمد: | 1476-5497 0307-0565 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc9767a8b367e822cc85fbd518617e5f https://doi.org/10.1038/ijo.2013.163 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....cc9767a8b367e822cc85fbd518617e5f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765497 03070565 |
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