This study was conducted to develop anin vitropropagation procedure forByblis linifloraSalisb. The process of seed sterilization andin vitrosowing achieved 95% efficiency. From the material aseptic stem segments, the shoot proliferation program was determined with the rate of 33.67 shoots/explants after 4 weeks of culture on nutrient medium (1/3 strength MS basal salts plus full Morel and Wetmore’s vitamins) with addition of a mixture of cytokinin (0.5 mg/L Kin and 2 mg/L BA) and then transferred to culture for another 4 weeks on nutrient medium. The rooting program from aseptic stem segments was to culture in nutrient medium supplemented with 0.5 mg/L NAA for 8 weeks. To produce a complete plant, single shoot separated from the proliferative clusters was transferred to a culture flask containing nutrient medium and cultured for 4 weeks. Thein vitroshoot developed more branches and self-rooted to form a complete plant. The suitable growing substrate in the acclimatization and outdoor stages was a mixture of peat moss and perlite (1 : 2 w/w). The climatic conditions of Ho Chi Minh City and the Southeast of Vietnam were suitable for growingByblis linifloratrees outdoors all year round.
