LiveLegionella pneumophilainduces MUC5AC production by airway epithelial cells independently of intracellular invasion
| العنوان: | LiveLegionella pneumophilainduces MUC5AC production by airway epithelial cells independently of intracellular invasion |
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| المؤلفون: | Koichi Yamada, Nobuko Araki, Hiroo Hasegawa, Yoshitomo Morinaga, Yosuke Harada, Tomoya Nishino, Shimeru Kamihira, Yoshihiro Yamamoto, Shigeru Kohno, Yohei Migiyama, Katsunori Yanagihara, Hiroshi Kakeya, Koichi Izumikawa, Kentaro Nagaoka |
| المصدر: | Canadian Journal of Microbiology. 58:151-157 |
| بيانات النشر: | Canadian Science Publishing, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | MAPK/ERK pathway, MAP Kinase Signaling System, media_common.quotation_subject, p38 mitogen-activated protein kinases, Immunology, Mucin 5AC, Applied Microbiology and Biotechnology, Microbiology, Legionella pneumophila, Immune System Phenomena, chemistry.chemical_compound, Genetics, Humans, Internalization, Molecular Biology, media_common, Cytochalasin D, biology, Mucin, NF-kappa B, Epithelial Cells, General Medicine, respiratory system, bacterial infections and mycoses, biology.organism_classification, respiratory tract diseases, chemistry, bacteria, Respiratory epithelium, Intracellular |
| الوصف: | The airway epithelium is the initial barrier against airborne pathogens, and it plays many roles in host airway defense. Legionella pneumophila is an intracellular pathogen that causes rapidly advancing pneumonia and is sometimes life-threatening. Here, we evaluated the role of the airway epithelial cells in the defense against L. pneumophila by examining mucus production in vitro. The production of MUC5AC, a major mucin protein, was not induced by formalin- or ultraviolet-killed L. pneumophila, but it was induced by live L. pneumophila. Similarly, nuclear factor-kappaB (NF-κB) was activated only by live L. pneumophila. Inhibitors of ERK and JNK, but not p38, dose-dependently inhibited the induction of MUC5AC by live L. pneumophila. Inhibition of intracellular invasion by cytochalasin D did not affect MUC5AC production. Taken together, the results suggest that live L. pneumophila induces MUC5AC production via the ERK-JNK and NF-κB pathways without internalization of bacteria and that the airway epithelium produces mucin as part of the immune response against L. pneumophila. |
| تدمد: | 1480-3275 0008-4166 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd39c4a508b46e06bfc6dd599cba3ff5 https://doi.org/10.1139/w11-123 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....cd39c4a508b46e06bfc6dd599cba3ff5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14803275 00084166 |
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