Quantum dot blueing and blinking enables fluorescence nanoscopy

التفاصيل البيبلوغرافية
العنوان: Quantum dot blueing and blinking enables fluorescence nanoscopy
المؤلفون: Patrick Hoyer, Johann Engelhardt, Stefan W. Hell, Thorsten Staudt
المصدر: Nano Letters
سنة النشر: 2010
مصطلحات موضوعية: GSD microscopy, Fluorescence-lifetime imaging microscopy, Nanostructure, Photon, Bioengineering, Sulfides, Microtubules, Sensitivity and Specificity, Cell Line, Optics, Microscopy, Quantum Dots, Fluorescence microscope, Cadmium Compounds, Animals, General Materials Science, Selenium Compounds, Stochastic Processes, Chemistry, business.industry, Mechanical Engineering, General Chemistry, Condensed Matter Physics, Photochemical Processes, Fluorescence, Spectrometry, Fluorescence, Quantum dot, Zinc Compounds, business
الوصف: We demonstrate superresolution fluorescence imaging of cells using bioconjugated CdSe/ZnS quantum dot markers. Fluorescence blueing of quantum dot cores facilitates separation of blinking markers residing closer than the diffraction barrier. The high number of successively emitted photons enables ground state depletion microscopy followed by individual marker return with a resolving power of the size of a single dot (∼12 nm). Nanoscale imaging is feasible with a simple webcam.
وصف الملف: application/pdf
تدمد: 1530-6992
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce141f3daf2a07af9307f4bef34f8e72
https://pubmed.ncbi.nlm.nih.gov/21128678
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....ce141f3daf2a07af9307f4bef34f8e72
قاعدة البيانات: OpenAIRE