Intra- and inter-protein couplings of backbone motions underlie protein thiol-disulfide exchange cascade
| العنوان: | Intra- and inter-protein couplings of backbone motions underlie protein thiol-disulfide exchange cascade |
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| المؤلفون: | Jienv Ding, Yunfei Hu, Xiaogang Niu, Changwen Jin, Wenbo Zhang |
| المصدر: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Arsenate Reductases, Arsenites, Stereochemistry, Science, 010402 general chemistry, 01 natural sciences, Article, 03 medical and health sciences, chemistry.chemical_compound, Thioredoxins, Bacterial Proteins, Disulfides, Sulfhydryl Compounds, CEST Experiments, Arsenite, chemistry.chemical_classification, Multidisciplinary, Chemical Exchange Saturation Transfer (CEST), Intermolecular force, Arsenate, 0104 chemical sciences, 030104 developmental biology, Enzyme, Arsenate reductase, CEST Data, chemistry, Catalytic cycle, Intramolecular force, Thiol-disulfide Exchange Reactions, Medicine, Arsenates, Thioredoxin, Arscia, Bacillus subtilis |
| الوصف: | The thioredoxin (Trx)-coupled arsenate reductase (ArsC) is a family of enzymes that catalyzes the reduction of arsenate to arsenite in the arsenic detoxification pathway. The catalytic cycle involves a series of relayed intramolecular and intermolecular thiol-disulfide exchange reactions. Structures at different reaction stages have been determined, suggesting significant conformational fluctuations along the reaction pathway. Herein, we use two state-of-the-art NMR methods, the chemical exchange saturation transfer (CEST) and the CPMG-based relaxation dispersion (CPMG RD) experiments, to probe the conformational dynamics of B. subtilis ArsC in all reaction stages, namely the enzymatic active reduced state, the intra-molecular C10–C82 disulfide-bonded intermediate state, the inactive oxidized state, and the inter-molecular disulfide-bonded protein complex with Trx. Our results reveal highly rugged energy landscapes in the active reduced state, and suggest global collective motions in both the C10–C82 disulfide-bonded intermediate and the mixed-disulfide Trx-ArsC complex. |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce144ee65cb6fd180b38d9daf01503dc https://doi.org/10.1038/s41598-018-33766-4 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ce144ee65cb6fd180b38d9daf01503dc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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