Negligible cytotoxicity induced by different titanium dioxide nanoparticles in fish cell lines
| العنوان: | Negligible cytotoxicity induced by different titanium dioxide nanoparticles in fish cell lines |
|---|---|
| المؤلفون: | Mona Connolly, María Luisa Fernández-Cruz, Azucena Bermejo-Nogales, Philipp Rosenkranz, José María Navas |
| المصدر: | Repositorio de Resultados de Investigación del INIA INIA: Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Carcinoma, Hepatocellular, Health, Toxicology and Mutagenesis, Cytotoxicity, Metal Nanoparticles, Topminnow fish, Nanotechnology, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, chemistry.chemical_compound, Lysosome, Cell Line, Tumor, medicine, Animals, Hepatocyte, 0105 earth and related environmental sciences, Nanomaterials, Titanium, Cell Membrane, Public Health, Environmental and Occupational Health, General Medicine, 021001 nanoscience & nanotechnology, Pollution, In vitro, Mitochondria, Rats, medicine.anatomical_structure, Rainbow trout, chemistry, Cell culture, Oncorhynchus mykiss, Titanium dioxide, Toxicity, Biophysics, Photocatalysis, Hepatocytes, 0210 nano-technology, Lysosomes, Hydrophobic and Hydrophilic Interactions, Function (biology) |
| الوصف: | Titanium dioxide nanoparticles (TiO2-NPs) have a wide number of applications in cosmetic, solar and paint industries due to their photocatalyst and ultraviolet blocking properties. The continuous increase in the production of TiO2-NPs enhances the risk for this manufactured nanomaterial to enter water bodies through treated effluents or agricultural amendments. TiO2-NPs have shown very low toxicity in a number of aquatic organisms. However, there are no conclusive data about their deleterious effects and on their possible mechanisms of toxic action. At this level, in vitro cell culture systems are a useful tool to gain insight about processes underlying the toxicity of a wide variety of substances, including nanomaterials. Differences in the physiology of different taxa make advisable the use of cells coming from the taxon of interest, but collecting data from a variety of cellular types allows a better understanding of the studied processes. Taking all this into account, the aim of the present study was to assess the toxicity of three types of TiO2-NP, rutile hydrophobic (NM-103), rutile hydrophilic (NM-104) and rutile-anatase (NM-105), obtained from the EU Joint Research Centre (JRC) repository, using various fish cell lines (RTG-2, PLHC-1, RTH-149, RTL-W1) and rainbow trout primary hepatocytes. For comparative purposes, the effect of different dispersion protocols, end-point assays and extended exposure time was studied in a fish cell line (RTG-2) and in the rat hepatoma cell line (H4IIE). TiO2-NPs dispersions showed a variable degree of aggregation in cell culture media. Disruption of mitochondrial metabolic activity, plasma membrane integrity and lysosome function was not detected in any cell line after exposure to TiO2-NPs at any time and concentration ranges tested. These results are indicative of a low toxicity of the TiO2-NPs tested and show the usefulness of fish cells maintained in vitro as high throughput screening methods that can facilitate further testing in the framework of integrated testing strategies. © 2017 Elsevier Inc. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce1c025f83e092b99a4e9d4ec424fa1b http://hdl.handle.net/20.500.12792/3574 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ce1c025f83e092b99a4e9d4ec424fa1b |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |