Development of a MD-LC-MS/MS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats
| العنوان: | Development of a MD-LC-MS/MS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats |
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| المؤلفون: | Chang Yang, Yahua Cui, Cai-Feng Liu, Jie Bai, Lin-Ying Zhong, Jun Liang, Daofang Liu, Shouying Du |
| المصدر: | Evidence-Based Complementary and Alternative Medicine, Vol 2019 (2019) Evidence-based Complementary and Alternative Medicine : eCAM |
| بيانات النشر: | Hindawi Limited, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0303 health sciences, Microdialysis, Chromatography, Article Subject, medicine.medical_treatment, Cmax, lcsh:Other systems of medicine, lcsh:RZ201-999, High-performance liquid chromatography, Eugenol, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Complementary and alternative medicine, chemistry, Pharmacokinetics, 030220 oncology & carcinogenesis, Piperine, medicine, Paeonol, Saline, Research Article, 030304 developmental biology |
| الوصف: | Huoxuezhitong rubber patch, a well-known traditional Chinese medicine (TCM) prescription, is utilized to treat pain and inflammation. In this study, a microdialysis-ultra-high-performance liquid chromatography-tandem mass spectrometry (MD-LC-MS/MS) method was designed for the simultaneous determination of active constituents in the rubber patch, such as paeonol (Pae), eugenol (Eug), and piperine (Pip). A microdialysis probe was implanted in the subcutaneous tissue of a rat, which is intended to detect the subcutaneous concentrations of target components. Saline containing 30% ethanol acted as perfusion fluid. Analytes in the microdialysate were completely separated over an ACQITY UPLC RBEH C18column (2.1mm×100mm, 1.7μm). The mobile phase was composed of 0.01% ammonia aqueous and acetonitrile-0.01% ammonia with gradient elution. The single-run analysis time was 10.0 minutes. The linear regression displayed good linear relationships in the ranges of 0.25–100 ng/mL for paeonol and eugenol and 0.001–5 ng/mL for piperine. The interday and intraday precision of the quality control samples exhibited relative standard deviations (RSD) Tmax(488.00±150.73) min was greater than that of Pae (186.67±48.44) min and Eug (240.00±138.56) min, and the rank order of t1/2was Pae > Pip > Eug. The rank order of AUC0-720andCmaxwas both Eug > Pae > Pip. MRT0-∞of Pip was higher than that of Pae and Eug. Eugenol showed a faster elimination and a shorter half-life. Paeonol showed a stronger drug reservoir function after removing the drug source. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 1741-4288 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cefac03e673aa96eddad356aa7de5c26 https://doaj.org/article/3bc7b8d5bb2945dbb8ddd23dba1d323a |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....cefac03e673aa96eddad356aa7de5c26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17414288 |
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