High Speed Ventral Plane Videography as a Convenient Tool to Quantify Motor Deficits during Pre-Clinical Experimental Autoimmune Encephalomyelitis
| العنوان: | High Speed Ventral Plane Videography as a Convenient Tool to Quantify Motor Deficits during Pre-Clinical Experimental Autoimmune Encephalomyelitis |
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| المؤلفون: | Felix Fischbach, Carsten Holzmann, Sebastian Rühling, Theresa Greiner, Vladislav Yakimov, Hannes Kaddatz, Julia Frenz, Jiangshan Zhan, Elena Nikolova, Markus Kipp, Sarah Joost |
| المصدر: | Cells Volume 8 Issue 11 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Encephalomyelitis, Autoimmune, Experimental, Functional impairment, Video Recording, experimental autoimmune encephalomyelitis, STRIDE, multiple sclerosis, Severity of Illness Index, Article, Lesion, Mice, Animal model, Animals, Medicine, business.industry, Multiple sclerosis, Experimental autoimmune encephalomyelitis, General Medicine, DigiGait™, medicine.disease, Gait, Mice, Inbred C57BL, Disease Models, Animal, Motor Skills, Rotarod Performance Test, Gait analysis, gait analysis, Female, Myelin-Oligodendrocyte Glycoprotein, medicine.symptom, business, Neuroscience |
| الوصف: | Experimental autoimmune encephalomyelitis (EAE) is the most commonly used multiple sclerosis animal model. EAE mice typically develop motor deficits in a caudal-to-rostral pattern when inflammatory lesions have already developed. However, to monitor more subtle behavioral deficits during lesion development (i.e., pre-clinical phase), more sophisticated methods are needed. Here, we investigated whether high speed ventral plane videography can be applied to monitor early motor deficits during &lsquo pre-clinical&rsquo EAE. For this purpose, EAE was induced in C57BL/6 mice and gait abnormalities were quantified using the DigiGait&trade apparatus. Gait deficits were related to histopathological changes. 10 out of 10 control (100%), and 14 out of 18 (77.8%) pre-clinical EAE mice could be evaluated using DigiGait&trade EAE severity was not influenced by DigiGait&trade related mice handlings. Most gait parameters recorded from day 6 post-immunization until the end of the experiment were found to be stable in control mice. During the pre-clinical phase, when conventional EAE scorings failed to detect any functional impairment, EAE mice showed an increased Swing Time, increased %Swing Stride, decreased %Stance Stride, decreased Stance/Swing, and an increased Absolute Paw Angle. In summary, DigiGait&trade is more sensitive than conventional scoring approaches to study motor deficits during the EAE pre-clinical phase. |
| وصف الملف: | application/pdf |
| تدمد: | 2073-4409 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf37329d35e2e8a1c3b4e0b1cf01e141 https://doi.org/10.3390/cells8111439 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....cf37329d35e2e8a1c3b4e0b1cf01e141 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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