Inhibition of HIV-1 infection by viral chemokine U83A via high-affinity CCR5 interactions that block human chemokine-induced leukocyte chemotaxis and receptor internalization
| العنوان: | Inhibition of HIV-1 infection by viral chemokine U83A via high-affinity CCR5 interactions that block human chemokine-induced leukocyte chemotaxis and receptor internalization |
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| المؤلفون: | D Dewin, Ursula A. Gompels, Chris M. Parry, Julie Catusse |
| المصدر: | Blood. 109:3633-3639 |
| بيانات النشر: | American Society of Hematology, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Receptors, CCR5, Chemokine receptor CCR5, viruses, media_common.quotation_subject, Immunology, C-C chemokine receptor type 6, CCR8, Biochemistry, Viral Proteins, Chemokine receptor, Chlorocebus aethiops, Animals, Humans, Protein Isoforms, CCL17, Internalization, media_common, Acquired Immunodeficiency Syndrome, Immunity, Cellular, biology, virus diseases, Cell Biology, Hematology, Molecular biology, Clathrin, Endocytosis, Alternative Splicing, Chemotaxis, Leukocyte, CCR5 Receptor Antagonists, COS Cells, HIV-1, biology.protein, XCL2, Receptors, Chemokine, Chemokines, Protein Binding, CCL21 |
| الوصف: | HIV-1 strains use C-C-chemokine receptor 5, CCR5, as a coreceptor for host transmission. Human CCR5 chemokine ligands inhibit binding and infection, whereas CCR5 mutations also inhibit infection by preventing surface expression, resulting in delayed progression to AIDS. Here, we describe a human herpesvirus 6 (HHV-6A) chemokine, U83A, which binds CCR5 with higher affinity than human chemokines, displacing their binding and leading to inhibition of chemotaxis of human leukocytes. Similarly, U83A inhibits infection by HIV-1 strains which use CCR5, but not the CXCR4, coreceptor. Unlike human CCR5 chemokine ligands which induce rapid CCR5 internalization mediated via clathrin, treatment with U83A prevents internalization. A spliced truncated U83A isoform, U83A-N, also binds CCR5 albeit with lower affinity, and this correlates with lower HIV-1 infection inhibition, whereas further truncation abolishes binding and any inhibition. Confocal microscopy confirms CCR5 internalization inhibition by U83A treatment, whereas labeled transferrin uptake shows that endocytosis via clathrin is unaltered. Previous results show that, although U83A-N is an antagonist, U83A is an agonist for CCR1, CCR4, CCR6, and CCR8 present on immune effector and antigen-presenting cells and here also shown for CCR5. Thus, U83A could act as a novel inhibitor of HIV-1 infection while also stimulating local immunity to the virus. |
| تدمد: | 1528-0020 0006-4971 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf7b7969114d9400b057462e2ee84312 https://doi.org/10.1182/blood-2006-08-042622 |
| رقم الأكسشن: | edsair.doi.dedup.....cf7b7969114d9400b057462e2ee84312 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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