Determination of kinetics and heat of hydrolysis for non-homogenous substrate by isothermal calorimetry
| العنوان: | Determination of kinetics and heat of hydrolysis for non-homogenous substrate by isothermal calorimetry |
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| المؤلفون: | Audrey Soric, Jean-Claude Sigoillot, Jean-Henry Ferrasse, Djida Tafoukt |
| المساهمون: | Laboratoire de Mécanique, Modélisation et Procédés Propres (M2P2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Biodiversité et Biotechnologie Fongiques (BBF), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Research Federation ECCOREV, Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM) |
| المصدر: | Bioprocess and Biosystems Engineering Bioprocess and Biosystems Engineering, 2017, 40 (4), pp.1-8. ⟨10.1007/s00449-016-1728-0⟩ Bioprocess and Biosystems Engineering, Springer Verlag, 2017, 40 (4), pp.1-8. ⟨10.1007/s00449-016-1728-0⟩ Bioprocess and Biosystems Engineering, Springer Verlag, 2017, pp.1-8. ⟨10.1007/s00449-016-1728-0⟩ |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Cellobiose dehydrogenase, Hot Temperature, Bioengineering, Cellulase, Calorimetry, 01 natural sciences, Isothermal process, 03 medical and health sciences, Hydrolysis, Biofuel, 010608 biotechnology, Enzymatic hydrolysis, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS, Triticum, biology, Ethanol, Chemistry, Substrate (chemistry), food and beverages, Isothermal titration calorimetry, General Medicine, Enzymes, Kinetics, 030104 developmental biology, Chemical engineering, Yield (chemistry), Biofuels, biology.protein, Carbohydrate Dehydrogenases, Biotechnology |
| الوصف: | International audience; The competitiveness of the second-generation bioethanol by biotechnological process requires an effective and quantitative control of biochemical reactions. In this study, the potential of isothermal calorimetry technique to measure heat and kinetics of a non-homogeneous substrate enzymatic hydrolysis is intended. Using this technique, optimum temperature of the enzymes used for lignocellulosic molecules hydrolysis was determined. Thus, the amount of substrate-to-enzyme ratio was highlighted as an important parameter of the hydrolysis yield. Furthermore, a new enzymes' cocktail efficiency consisting of a mix of cellulases and cellobiose dehydrogenase (CDH) was qualified by this technique. The results showed that this cocktail allowed the production of a high amount of gluconic acid that could improve the attractiveness of these second-generation biofuels. From the set of experiments, the hydrolysis heat of wheat straw was derived and a meaningful value of -32.2 +/- 3.2 J g(-1) (gram reducing sugars product) is calculated. Then, isothermal measurements were used to determine kinetic constants of the cellulases and CDH mix on wheat straw. Results showed that this enzyme cocktail has an optimal rate at 45 A degrees C in the range of temperatures tested (40-55 A degrees C). |
| تدمد: | 1615-7605 1615-7591 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d012c4cada9d90fa49ecad5d266ed3f0 https://pubmed.ncbi.nlm.nih.gov/28062914 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....d012c4cada9d90fa49ecad5d266ed3f0 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16157605 16157591 |
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