Altered Expression Profile of IgLON Family of Neural Cell Adhesion Molecules in the Dorsolateral Prefrontal Cortex of Schizophrenic Patients
| العنوان: | Altered Expression Profile of IgLON Family of Neural Cell Adhesion Molecules in the Dorsolateral Prefrontal Cortex of Schizophrenic Patients |
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| المؤلفون: | Karina Karis, Kattri-Liis Eskla, Maria Kaare, Karin Täht, Jana Tuusov, Tanel Visnapuu, Jürgen Innos, Mohan Jayaram, Tõnis Timmusk, Cynthia S. Weickert, Marika Väli, Eero Vasar, Mari-Anne Philips |
| المصدر: | Frontiers in Molecular Neuroscience Frontiers in Molecular Neuroscience, Vol 11 (2018) European Union Open Data Portal DOAJ-Articles PubMed Central Sygma Frontiers UnpayWall ORCID Microsoft Academic Graph |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Neurite, business.industry, Cell adhesion molecule, medicine.disease, lcsh:RC321-571, Dorsolateral prefrontal cortex, 03 medical and health sciences, Cellular and Molecular Neuroscience, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Schizophrenia, Endophenotype, Synaptic plasticity, Medicine, Neural cell adhesion molecule, business, Molecular Biology, Neuroscience, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, 030217 neurology & neurosurgery, Diagnosis of schizophrenia, Original Research |
| الوصف: | Neural adhesion proteins are crucial in the development and maintenance of functional neural connectivity. Growing evidence suggests that the IgLON family of neural adhesion molecules LSAMP, NTM, NEGR1, and OPCML are important candidates in forming the susceptibility to schizophrenia (SCZ). IgLON proteins have been shown to be involved in neurite outgrowth, synaptic plasticity and neuronal connectivity, all of which have been shown to be altered in the brains of patients with the diagnosis of schizophrenia. Here we optimized custom 5'-isoform-specific TaqMan gene-expression analysis for the transcripts of human IgLON genes to study the expression of IgLONs in the dorsolateral prefrontal cortex (DLPFC) of schizophrenic patients (n = 36) and control subjects (n = 36). Uniform 5'-region and a single promoter was confirmed for the human NEGR1 gene by in silico analysis. IgLON5, a recently described family member, was also included in the study. We detected significantly elevated levels of the NEGR1 transcript (1.33-fold increase) and the NTM 1b isoform transcript (1.47-fold increase) in the DLPFC of schizophrenia patients compared to healthy controls. Consequent protein analysis performed in male subjects confirmed the increase in NEGR1 protein content both in patients with the paranoid subtype and in patients with other subtypes. In-group analysis of patients revealed that lower expression of certain IgLON transcripts, mostly LSAMP 1a and 1b, could be related with concurrent depressive endophenotype in schizophrenic patients. Additionally, our study cohort provides further evidence that cannabis use may be a relevant risk factor associated with suicidal behaviors in psychotic patients. In conclusion, we provide clinical evidence of increased expression levels of particular IgLON family members in the DLPFC of schizophrenic patients. We propose that alterations in the expression profile of IgLON neural adhesion molecules are associated with brain circuit disorganization in neuropsychiatric disorders, such as schizophrenia. In the light of previously published data, we suggest that increased level of NEGR1 in the frontal cortex may serve as molecular marker for a wider spectrum of psychiatric conditions. |
| تدمد: | 1662-5099 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d09563833929f16e07c8ba40df90f785 https://pubmed.ncbi.nlm.nih.gov/29434535 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d09563833929f16e07c8ba40df90f785 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16625099 |
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