Pattern of muscle fiber type formation in the pig
العنوان: | Pattern of muscle fiber type formation in the pig |
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المؤلفون: | Patrick Ecolan, F. Edom, Louis Lefaucheur, G. S. Butler-Browne |
المساهمون: | Station de recherches porcines, Institut National de la Recherche Agronomique (INRA), Inconnu, ProdInra, Migration |
المصدر: | Developmental Dynamics Developmental Dynamics, Wiley, 1995, 203, pp.27-41 |
بيانات النشر: | Wiley, 1995. |
سنة النشر: | 1995 |
مصطلحات موضوعية: | Gene isoform, Swine, Gestational Age, Myosins, Muscle Development, Major histocompatibility complex, 03 medical and health sciences, [SDV.BDD] Life Sciences [q-bio]/Development Biology, Myosin, medicine, Animals, Myocyte, Muscle, Skeletal, [SDV.BDD]Life Sciences [q-bio]/Development Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Fetus, biology, Myogenesis, Myocardium, 0402 animal and dairy science, Antibodies, Monoclonal, Gene Expression Regulation, Developmental, Skeletal muscle, 04 agricultural and veterinary sciences, Immunohistochemistry, 040201 dairy & animal science, Molecular biology, medicine.anatomical_structure, Animals, Newborn, biology.protein, Developmental Biology |
الوصف: | The aim of this study was to analyze the temporal sequence of expression of the myosin isoforms in the populations of muscle fibers in the pig and to bring more information on the origin of the strikingly different pattern of fiber composition and distribution between the deep medial red (oxido-glycolytic) and superficial white (glycolytic) portions of semitendinosus (ST) muscle. Muscle samples were taken from 49-, 55-, 75-, 90-, 103-, and 113- (birth) day-old fetuses, from 6-, 11-, 21-, 35-, 50-, and 80-day-old piglets, and from a 3-year-old pig. Our results confirm the sequential formation of primary and secondary generation fibers. The use of immunohistochemistry and heterologous monoclonal antibodies (mAb) directed against specific myosin heavy chain (MHC) isoforms revealed a different pattern of gene expression between the two portions of the ST muscle for both generations of fibers. By 75 days of gestation (dg), primary myotubes from the deep medial portion stained positively for the anti-slow MHC mAb and negatively for the adult anti-fast MHC, whereas the opposite was observed in the superficial portion. Secondary fibers never expressed slow MHC until late gestation. Instead, they expressed an adult fast MHC isoform as soon as they formed in the deep medial portion and later on in the superficial portion. From late gestation to the first 3 postnatal weeks, slow MHC began to be expressed in a subpopulation of secondary fibers. These fibers were in the direct vicinity of primary myotubes in the deep medial portion, whereas their location could not be established in the superficial portion. The remaining secondary fibers matured to type IIA in the direct vicinity of these type I fibers and to type IIB at the periphery of the islets. In both portions of the muscle, a subpopulation of secondary fibers, the first ones to express slow MHC, also transitorily expressed a MHC that was identical or closely related to the alpha-cardiac MHC during the early postnatal period. A third generation of small diameter fibers was observed shortly after birth and reacted with the anti-fetal MHC mAb; their destiny remains to be established.(ABSTRACT TRUNCATED AT 400 WORDS) |
تدمد: | 1058-8388 1097-0177 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d13361b7be0febb6d160e40dcba0a1fd https://doi.org/10.1002/aja.1002030104 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....d13361b7be0febb6d160e40dcba0a1fd |
قاعدة البيانات: | OpenAIRE |
تدمد: | 10588388 10970177 |
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