Melting Transition of Oriented DNA Fibers Submerged in Poly(ethylene glycol) Solutions Studied by Neutron Scattering and Calorimetry
| العنوان: | Melting Transition of Oriented DNA Fibers Submerged in Poly(ethylene glycol) Solutions Studied by Neutron Scattering and Calorimetry |
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| المؤلفون: | Michel Peyrard, Estelle Mossou, Adrián González, Nikos Theodorakopoulos, Andrew J Studer, Santiago Cuesta-Lopez, Gaël Moiroux, Marta Marty-Roda, Jean-Luc Garden, Bruno Demé, Andrew Wildes |
| المساهمون: | Institut Laue-Langevin (ILL), ILL, ICCRAM, University of Burgos, ICCRAM, University of Burgos (ICCRAM), Keele Univ, Fac Nat Sci, Keele ST5 5BG, Staffs, England, ANSTO, Thermique Elaboration Matériaux Applications (ThEMA), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Theoretical and Physical Chemistry Institute NHRF, National Hellenic Research Foundation, Laboratoire de Physique de l'ENS Lyon (Phys-ENS), École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Universidad de Burgos, Keele University [Keele], Epitaxie et couches minces (NEEL- EpiCM), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), National Hellenic Research Foundation [Athens], École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Journal of Physical Chemistry B Journal of Physical Chemistry B, American Chemical Society, 2018, 122 (9), pp.2504-2515. ⟨10.1021/acs.jpcb.7b11608⟩ Journal of Physical Chemistry B, 2018, 122 (9), pp.2504-2515. ⟨10.1021/acs.jpcb.7b11608⟩ |
| بيانات النشر: | American Chemical Society (ACS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Materials science, Analytical chemistry, Bragg peak, Calorimetry, Neutron scattering, 01 natural sciences, Polyethylene Glycols, 03 medical and health sciences, chemistry.chemical_compound, Salmon, 0103 physical sciences, Materials Chemistry, Animals, Scattering, Radiation, Transition Temperature, Osmotic pressure, Neutron, [PHYS.COND.CM-SM]Physics [physics]/Condensed Matter [cond-mat]/Statistical Mechanics [cond-mat.stat-mech], Physical and Theoretical Chemistry, 010306 general physics, ComputingMilieux_MISCELLANEOUS, Neutrons, Calorimetry, Differential Scanning, Transition temperature, Intermolecular force, DNA, Surfaces, Coatings and Films, Solutions, 030104 developmental biology, chemistry, Ethylene glycol |
| الوصف: | The influence of molecular confinement on the melting transition of oriented Na-DNA fibers submerged in poly(ethylene glycol) (PEG) solutions has been studied. The PEG solution exerts an osmotic pressure on the fibers which, in turn, is related to the DNA intermolecular distance. Calorimetry measurements show that the melting temperature increases and the width of the transition decreases with decreasing intermolecular distance. Neutron scattering was used to monitor the integrated intensity and width of a Bragg peak from the B-form of DNA as a function of temperature. The data were quantitatively analyzed using the Peyrard-Bishop-Dauxois model. The experiments and analysis showed that long segments of double-stranded DNA persist until the last stages of melting and that there appears to be a substantial increase of the DNA dynamics as the melting temperature of the DNA is approached. |
| تدمد: | 1520-5207 1520-6106 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d13be2ffabda2ea7f5446fece10b5f4b https://doi.org/10.1021/acs.jpcb.7b11608 |
| رقم الأكسشن: | edsair.doi.dedup.....d13be2ffabda2ea7f5446fece10b5f4b |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15205207 15206106 |
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