Aberrant miR‐145–5p/β‐catenin signal impairs osteocyte function in adolescent idiopathic scoliosis
| العنوان: | Aberrant miR‐145–5p/β‐catenin signal impairs osteocyte function in adolescent idiopathic scoliosis |
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| المؤلفون: | Bobby K. W. Ng, Jack C. Y. Cheng, Wayne Yuk-Wai Lee, Huanxiong Chen, Ross Ka Kit Leung, Tsz Ping Lam, Jiajun Zhang, Jian Q. Feng, Yong Qiu, Kwong Wai Choy |
| المصدر: | The FASEB Journal. 32:6537-6549 |
| بيانات النشر: | Wiley, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Gene knockdown, biology, business.industry, Microarray analysis techniques, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, Circulating MicroRNA, 030104 developmental biology, medicine.anatomical_structure, chemistry, Osteoprotegerin, Osteocyte, microRNA, Genetics, Cancer research, biology.protein, Medicine, Sclerostin, Osteopontin, business, Molecular Biology, Biotechnology |
| الوصف: | Recently, noncoding RNAs have been thought to play important roles in the sporadic occurrence of spinal deformity of adolescent idiopathic scoliosis (AIS). As a prognostic factor for curve progression, low bone mass has been hypothesized to crosstalk with AIS pathogenesis. Abnormal osteoblasts activities are reported in AIS without a clear mechanism. In this study, bone biopsies from patients with AIS and control subjects and the primary osteoblasts derived from those samples were used to identify the potential microRNA (miRNA) candidates that interfere with osteoblasts and osteocytes function. Microarray analysis identified miRNA-145-5p (miR-145) as a potential upstream regulator. miR-145 and β-catenin mRNA ( CTNNB1) were overexpressed in AIS bone tissues and primary osteoblasts, and their expression correlated positively in AIS. Knockdown of miR-145 restored impaired osteocyte activity through the down-regulation of active β-catenin expression and its transcriptional activity. Significant negative correlations between circulating miR-145 and serum sclerostin, osteopontin, and osteoprotegerin were noted in patients with AIS, which was in line with our cellular findings. This is the first study to demonstrate the effect of aberrant miRNA expression and its effect on osteocyte function in AIS, which may contribute to the low bone mass. Our findings also provide insight into the development of circulating microRNAs as a bone quality biomarker or even a prognostic biomarker for AIS.-Zhang, J., Chen, H., Leung, R. K. K., Choy, K. W., Lam, T. P., Ng, B. K. W., Qiu,Y., Feng, J. Q., Cheng, J. C. Y., Lee, W. Y. W. Aberrant miR-145-5p/β-catenin signal impairs osteocyte function in adolescent idiopathic scoliosis. |
| تدمد: | 1530-6860 0892-6638 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1efa6be77b53ace723c9d2e700c1612 https://doi.org/10.1096/fj.201800281 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d1efa6be77b53ace723c9d2e700c1612 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15306860 08926638 |
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