A novel application of Gini coefficient for the quantitative measurement of bacterial aggregation
| العنوان: | A novel application of Gini coefficient for the quantitative measurement of bacterial aggregation |
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| المؤلفون: | Zhi-zhong Wang, Yu-ming Cai, Robert P. Howlin, David S. Chatelet, Jeremy S. Webb |
| المصدر: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Adolescent, Cystic Fibrosis, 030106 microbiology, Population, lcsh:Medicine, Bacterial population, Clinical settings, Nitric Oxide, Microbiology, Bacterial Adhesion, Article, Cell size, 03 medical and health sciences, Young Adult, Imaging, Three-Dimensional, Average size, Humans, education, lcsh:Science, education.field_of_study, Multidisciplinary, Gini coefficient, Chemistry, Aggregate (data warehouse), lcsh:R, Middle Aged, Plankton, Computational biology and bioinformatics, 030104 developmental biology, Biofilms, Pseudomonas aeruginosa, lcsh:Q, Biological system |
| الوصف: | Non-surface attached bacterial aggregates are frequently found in clinical settings associated with chronic infections. Current methods quantifying the extent to which a suspended bacterial population is aggregated mainly rely on: (1) cell size distribution curves that are difficult to be compared numerically among large-scale samples; (2) the average size/proportion of aggregates in a population that do not specify the aggregation patterns. Here we introduce a novel application of Gini coefficient, herein named Aggregation Coefficient (AC), to quantify the aggregation levels of cystic fibrosis Pseudomonas aeruginosa (CF-PA) isolates in vitro using 3D micrographs, Fiji and MATLAB. Different aggregation patterns of five strains were compared statistically using the numerical AC indexes, which correlated well with the size distribution curves plotted by different biovolumes of aggregates. To test the sensitivity of AC, aggregates of the same strains were treated with nitric oxide (NO), a dispersal agent that reduces the biomass of surface attached biofilms. Strains unresponsive to NO were reflected by comparable AC indexes, while those undergoing dispersal showed a significant reduction in AC index, mirroring the changes in average aggregate sizes and proportions. Therefore, AC provides simpler and more descriptive numerical outputs for measuring different aggregation patterns compared to current approaches. |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1f1eaa890c2284c72c917387683ed15 https://pubmed.ncbi.nlm.nih.gov/31831832 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d1f1eaa890c2284c72c917387683ed15 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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