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An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper®

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العنوان: An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper®
المؤلفون: Fraser Symmans, Arndt Hartmann, Andreas Turzynski, Claudia Gürtler, Peter Sinn, Robert Stoehr, Zsuzsanna Varga, Reinhard von Wasielewski, Mark Laible, Ugur Sahin, Frédérique Penault-Llorca, John M. S. Bartlett, Annette Lebeau, Hong Bu, Peter Schraml, Elena Guerini-Rocco, Thomas Keller, Heikki Joensuu, Kornelia Schlombs, Xiaodong Teng, Giuseppe Viale
المساهمون: University of Zurich, Varga, Zsuzsanna, Institute for Veterinary Medical Research [Budapest] (AOTI), Centre for Agricultural Research [Budapest] (ATK), Hungarian Academy of Sciences (MTA)-Hungarian Academy of Sciences (MTA), Institute of Pathology, Universität Regensburg (UR), Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), University hospital of Zurich [Zurich], Department of Pediatrics, Josefinum Augsburg, Augsburg, Division of Pathology and Laboratory Medicine, Università degli Studi di Milano [Milano] (UNIMI)-European Institute of Oncology [Milan] (ESMO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Università degli Studi di Milano = University of Milan (UNIMI)-European Institute of Oncology [Milan] (ESMO), Clinicum, Heikki Joensuu / Principal Investigator, Department of Oncology
المصدر: Breast Cancer Research
Breast Cancer Research, BioMed Central, 2017, 19 (1), pp.55. ⟨10.1186/s13058-017-0848-z⟩
Breast Cancer Research, Vol 19, Iss 1, Pp 1-13 (2017)
Breast Cancer Research : BCR
سنة النشر: 2017
مصطلحات موضوعية: 0301 basic medicine, Oncology, Pathology, Receptor, ErbB-2, Receptor expression, Estrogen receptor, FFPE, MKI67, RECOMMENDATIONS, 610 Medical sciences Medicine, 0302 clinical medicine, Cohen's kappa, Breast cancer, Medicine, 1306 Cancer Research, Prospective Studies, skin and connective tissue diseases, ERBB2, KI67, AMERICAN-SOCIETY, DISTANT RECURRENCE, ESR1, Intracellular Signaling Peptides and Proteins, RECEPTOR EXPRESSION, Nuclear Proteins, Prognosis, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Reproducibility, 3. Good health, Gene Expression Regulation, Neoplastic, 030220 oncology & carcinogenesis, PGR, Female, 2730 Oncology, Research Article, medicine.medical_specialty, 3122 Cancers, Breast Neoplasms, [SDV.CAN]Life Sciences [q-bio]/Cancer, 610 Medicine & health, lcsh:RC254-282, 03 medical and health sciences, Formaldehyde, Internal medicine, 10049 Institute of Pathology and Molecular Pathology, Progesterone receptor, Biomarkers, Tumor, Humans, RNA, Messenger, RECURRENCE SCORE, ENDOCRINE THERAPY, business.industry, RT-qPCR, Estrogen Receptor alpha, WORKING GROUP, medicine.disease, Ki-67 Antigen, ESTROGEN-RECEPTOR, 030104 developmental biology, MammaTyper, business, Estrogen receptor alpha, Kappa
الوصف: Background Accurate determination of the predictive markers human epidermal growth factor receptor 2 (HER2/ERBB2), estrogen receptor (ER/ESR1), progesterone receptor (PgR/PGR), and marker of proliferation Ki67 (MKI67) is indispensable for therapeutic decision making in early breast cancer. In this multicenter prospective study, we addressed the issue of inter- and intrasite reproducibility using the recently developed reverse transcription-quantitative real-time polymerase chain reaction-based MammaTyper® test. Methods Ten international pathology institutions participated in this study and determined messenger RNA expression levels of ERBB2, ESR1, PGR, and MKI67 in both centrally and locally extracted RNA from formalin-fixed, paraffin-embedded breast cancer specimens with the MammaTyper® test. Samples were measured repeatedly on different days within the local laboratories, and reproducibility was assessed by means of variance component analysis, Fleiss’ kappa statistics, and interclass correlation coefficients (ICCs). Results Total variations in measurements of centrally and locally prepared RNA extracts were comparable; therefore, statistical analyses were performed on the complete dataset. Intersite reproducibility showed total SDs between 0.21 and 0.44 for the quantitative single-marker assessments, resulting in ICC values of 0.980–0.998, demonstrating excellent agreement of quantitative measurements. Also, the reproducibility of binary single-marker results (positive/negative), as well as the molecular subtype agreement, was almost perfect with kappa values ranging from 0.90 to 1.00. Conclusions On the basis of these data, the MammaTyper® has the potential to substantially improve the current standards of breast cancer diagnostics by providing a highly precise and reproducible quantitative assessment of the established breast cancer biomarkers and molecular subtypes in a decentralized workup. Electronic supplementary material The online version of this article (doi:10.1186/s13058-017-0848-z) contains supplementary material, which is available to authorized users.
وصف الملف: application/pdf
اللغة: English
تدمد: 1465-5411
1465-542X
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d234172e9dc719482f3a3eb9925232f5
https://doi.org/10.5167/uzh-137106
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....d234172e9dc719482f3a3eb9925232f5
قاعدة البيانات: OpenAIRE
الوصف
تدمد:14655411
1465542X