Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice
| العنوان: | Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice |
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| المؤلفون: | Jianfei Ma, Tianhua Xu, Li Yao, Li Sun, Changlong Lv, Danan Wang, Lining Wang, Jiangmin Feng, Quan Yuan |
| المصدر: | Biotechnology Letters. 39:1159-1166 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Recombinant Fusion Proteins, T cell, Bioengineering, Biology, Applied Microbiology and Biotechnology, DNA vaccination, Interferon-gamma, Mice, 03 medical and health sciences, 0302 clinical medicine, Immune system, Adjuvants, Immunologic, Antigen, Vaccines, DNA, medicine, Animals, Humans, Tuberculosis, Interferon gamma, Cloning, Molecular, Tuberculosis Vaccines, Cells, Cultured, Antigens, Bacterial, Interleukin, General Medicine, Virology, Mice, Inbred C57BL, HEK293 Cells, 030104 developmental biology, medicine.anatomical_structure, Interleukin 15, Immunology, Tuberculosis vaccines, Bronchoalveolar Lavage Fluid, Acyltransferases, Spleen, Plasmids, 030215 immunology, Biotechnology, medicine.drug |
| الوصف: | To investigate the potential of interleukin (IL)-15 as a novel adjuvant for Mycobacterium tuberculosis (Mtb) antigen 85A (Ag85A) vaccine. C57BL/6 mice were intramuscularly immunized three times with a plasmid expressing the Ag85A-IL-15 fusion protein (pcDNA3.1-Ag85A-IL-15), with the empty pcDNA3.1 vector and the pcDNA3.1-Ag85A as control. Mice vaccinated with pcDNA3.1-Ag85A-IL-15 generated more secretory IgA (sIgA) into their lung (209 ± 21 μg/ml) and acquired an enhanced serum IgG response to Ag85A. IgG2a/IgG1 ratios were upregulated, natural killer cell activity was augmented and Ag85A-specific splenic T cell proliferation was enhanced in these mice as well. Vaccination with pcDNA3.1-Ag85A-IL-15 promoted the polarization of CD4+ T cells towards a Th1 type in the spleen, and significantly upregulated the serum level of interferon (IFN)-γ (458 ± 98 pg/ml), a typical Th1 cytokine. IFN-γ-expressing CD8+ cells were also increased in the spleen after pcDNA3.1-Ag85A-IL-15 immunization. A superior immune type I response in mice vaccinated with plasmid Ag85A-IL-15 has been achieved. |
| تدمد: | 1573-6776 0141-5492 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2b25efdda34e0999b1366cd75c12ddc https://doi.org/10.1007/s10529-017-2342-1 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....d2b25efdda34e0999b1366cd75c12ddc |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15736776 01415492 |
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