Neutralization of Junín virus by single domain antibodies targeted against the nucleoprotein
| العنوان: | Neutralization of Junín virus by single domain antibodies targeted against the nucleoprotein |
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| المؤلفون: | Paco Hulpiau, Xavier Saelens, Ioanna Christopoulou, Claudia Soledad Sepúlveda, Florencia N. Linero, Luis Alberto Scolaro |
| المصدر: | Scientific Reports SCIENTIFIC REPORTS Scientific Reports, Vol 8, Iss 1, Pp 1-17 (2018) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, viruses, lcsh:Medicine, Neutralization, purl.org/becyt/ford/1 [https], Virion binding, Antibody Specificity, Medicine and Health Sciences, LASSA FEVER, lcsh:Science, Lassa fever, Neutralizing, Multidisciplinary, Attenuated vaccine, LIVE-ATTENUATED VACCINE, Membrane Glycoproteins, biology, GLYCOPROTEIN, Arenavirus, ARGENTINE HEMORRHAGIC-FEVER, INCLUSION-BODY DISEASE, CIENCIAS NATURALES Y EXACTAS, ARENAVIRUS INFECTIONS, Argentine hemorrhagic fever, Article, Ciencias Biológicas, CANDID NUMBER-1, 03 medical and health sciences, Viral Proteins, medicine, Animals, Point Mutation, Amino Acid Sequence, purl.org/becyt/ford/1.6 [https], VHHs, Junin virus, RECEPTOR, lcsh:R, Biology and Life Sciences, Viral Vaccines, IN-VITRO, Single-Domain Antibodies, Junín, medicine.disease, biology.organism_classification, Virology, Antibodies, Neutralizing, Nucleoprotein, LYMPHOCYTIC CHORIOMENINGITIS VIRUS, 030104 developmental biology, Nucleoproteins, lcsh:Q, Virología |
| الوصف: | The syndrome viral haemorrhagic fever (VHF) designates a broad range of diseases that are caused by different viruses including members of the family Arenaviridae. Prophylaxis for Argentine Haemorrhagic Fever (AHF), caused by the arenavirus Junín (JUNV), has been achieved by the use of a live attenuated vaccine, named Candid#1. The standard treatment of AHF is transfusion of convalescent human plasma. Our aim was to develop an alternative and safer treatment for AHF based on the use of virus-neutralizing single domain antibodies (VHHs). We describe the first reported VHHs directed against an arenavirus. These VHHs could neutralize Candid#1 by altering virion binding/fusion. Surprisingly, the neutralizing VHHs appeared to be specific for the viral nucleoprotein (N) that is not known to be involved in arenavirus entry. Candid#1 VHH-escape viruses had acquired a predicted N-glycosylation site in the surface glycoprotein GP1 that is present in highly pathogenic JUNV strains. Accordingly, the Candid#1-neutralizing VHHs could not neutralize pathogenic JUNV strains, but they could still bind to cells infected with a pathogenic strain or the escape mutant viruses. These results show that the attenuated strains of JUNV can be potently neutralized by nucleoprotein-specific VHHs. Fil: Linero, Florencia Natalia. Flanders Interuniversity Institute For Biotechnology; Bélgica. University of Ghent; Bélgica Fil: Sepúlveda, Claudia Soledad. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Christopoulou, Ioanna. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; Bélgica Fil: Hulpiau, Paco. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; Bélgica Fil: Scolaro, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina Fil: Saelens, Xavier. University of Ghent; Bélgica. Flanders Interuniversity Institute For Biotechnology; Bélgica |
| وصف الملف: | application/pdf |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2b74c19f6392e5a410d97007861f2b9 https://pubmed.ncbi.nlm.nih.gov/30061671 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d2b74c19f6392e5a410d97007861f2b9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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