REGULATION OF MELANOGENESIS BY THE AMINO-ACID TRANSPORTER SLC7A5
| العنوان: | REGULATION OF MELANOGENESIS BY THE AMINO-ACID TRANSPORTER SLC7A5 |
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| المؤلفون: | Corine Bertolotto, Céline Gaudel, Justine Leclerc, Karine Bille, Robert Ballotti, Frédéric Soysouvanh, François Montcriol, Chrystel Husser |
| المساهمون: | Nunii Laboratoire / Grasse Biotech (Grasse, France), Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), This work was supported by grants from INSERM, the University of Nice-Sophia-Antipolis and Nunni laboratoire., BERTOLOTTO-BALLOTTI, BERTOLOTTO-BALLOTTI, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | Journal of Investigative Dermatology Journal of Investigative Dermatology, Nature Publishing Group, 2020, Epub ahead of print. ⟨10.1016/j.jid.2020.03.941⟩ |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Small interfering RNA, Tyrosinase, Carboxylic Acids, 4-dihydroxyphenylalanine, [SDV.CAN]Life Sciences [q-bio]/Cancer, Dermatology, tyrosinase, Biochemistry, Large Neutral Amino Acid-Transporter 1, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, [SDV.CAN] Life Sciences [q-bio]/Cancer, Cell Line, Tumor, medicine, tyrosine-related protein 1, Animals, Humans, Amino acid transporter, RNA, Small Interfering, Molecular Biology, Melanins, Microphthalmia-Associated Transcription Factor, Gene knockdown, Epidermis (botany), dopachrome tautomerase, Pigmentation, Chemistry, Cell Biology, Norbornanes, Hyperpigmentation, L-3, 3. Good health, 030104 developmental biology, Pyrones, Cell culture, 030220 oncology & carcinogenesis, medicine.symptom, Kojic acid |
| الوصف: | Integration of chromatin immunoprecipitation-sequencing and microarray data enabled us to identify previously unreported MITF-target genes, among which the amino acid transporter SLC7A5 is also included. We reported that small interfering RNA-mediated SLC7A5 knockdown decreased pigmentation in B16F10 cells but neither affected morphology nor dendricity. Treatment with the SLC7A5 inhibitors 2-amino-2-norbornanecarboxylic acid (BCH) or JPH203 also decreased melanin synthesis in B16F10 cells. Our findings indicated that BCH was as potent as reference depigmenting agent, kojic acid, but acted through a different pathway not affecting tyrosinase activity. BCH also decreased pigmentation in human MNT1 melanoma cells or normal human melanocytes. Finally, we tested BCH on a more physiological model, using reconstructed human epidermis and confirmed a strong inhibition of pigmentation, revealing the clinical potential of SLC7A5 inhibition and positioning BCH as a depigmenting agent suitable for cosmetic or dermatological intervention in hyperpigmentation diseases. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0022-202X 1523-1747 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2e23fe72de1913046909de367d77eef https://www.hal.inserm.fr/inserm-02529683 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d2e23fe72de1913046909de367d77eef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0022202X 15231747 |
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