SorCS1-mediated sorting in dendrites maintains neurexin axonal surface polarization required for synaptic function
| العنوان: | SorCS1-mediated sorting in dendrites maintains neurexin axonal surface polarization required for synaptic function |
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| المؤلفون: | Julie Nys, Ben Verpoort, Joris de Wit, Kristel M. Vennekens, Keimpe D. Wierda, Luís Ribeiro |
| المصدر: | PLoS Biology PLoS Biology, Vol 17, Iss 10, p e3000466 (2019) |
| بيانات النشر: | Public Library of Science, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, B Vitamins, Neurexin, Endoplasmic Reticulum, Biochemistry, Synaptic Transmission, Synapse, Mice, 0302 clinical medicine, Nerve Fibers, Postsynaptic potential, Animal Cells, Cell polarity, Protein Isoforms, Axon, Biology (General), Neural Cell Adhesion Molecules, Neurons, Cerebral Cortex, Secretory Pathway, Organic Compounds, General Neuroscience, Cell Polarity, Vitamins, Endocytosis, Transport protein, Cell biology, Chemistry, Protein Transport, medicine.anatomical_structure, Cell Processes, Physical Sciences, Cellular Types, Cellular Structures and Organelles, General Agricultural and Biological Sciences, Research Article, Endosome, QH301-705.5, Imaging Techniques, Primary Cell Culture, Synaptic Membranes, Biotin, Receptors, Cell Surface, Endosomes, Biology, Research and Analysis Methods, Green Fluorescent Protein, General Biochemistry, Genetics and Molecular Biology, Mitochondrial Proteins, 03 medical and health sciences, Fluorescence Imaging, medicine, Animals, Humans, Vesicles, Rats, Wistar, General Immunology and Microbiology, Organic Chemistry, Calcium-Binding Proteins, Chemical Compounds, Biology and Life Sciences, Proteins, Cell Biology, Neuronal Dendrites, Embryo, Mammalian, Axons, Rats, Mice, Inbred C57BL, Luminescent Proteins, 030104 developmental biology, HEK293 Cells, Gene Expression Regulation, rab GTP-Binding Proteins, Cellular Neuroscience, Neuron, 030217 neurology & neurosurgery, Neuroscience |
| الوصف: | The pre- and postsynaptic membranes comprising the synaptic junction differ in protein composition. The membrane trafficking mechanisms by which neurons control surface polarization of synaptic receptors remain poorly understood. The sorting receptor Sortilin-related CNS expressed 1 (SorCS1) is a critical regulator of trafficking of neuronal receptors, including the presynaptic adhesion molecule neurexin (Nrxn), an essential synaptic organizer. Here, we show that SorCS1 maintains a balance between axonal and dendritic Nrxn surface levels in the same neuron. Newly synthesized Nrxn1α traffics to the dendritic surface, where it is endocytosed. Endosomal SorCS1 interacts with the Rab11 GTPase effector Rab11 family-interacting protein 5 (Rab11FIP5)/Rab11 interacting protein (Rip11) to facilitate the transition of internalized Nrxn1α from early to recycling endosomes and bias Nrxn1α surface polarization towards the axon. In the absence of SorCS1, Nrxn1α accumulates in early endosomes and mispolarizes to the dendritic surface, impairing presynaptic differentiation and function. Thus, SorCS1-mediated sorting in dendritic endosomes controls Nrxn axonal surface polarization required for proper synapse development and function. The membrane trafficking mechanisms that regulate the polarized distribution of synaptic receptors in neurons are poorly understood. This study shows that endosomal sorting in dendrites controls the axonal surface polarization of the synaptic organizer neurexin, which is required for proper presynaptic differentiation and function. |
| وصف الملف: | Electronic-eCollection |
| اللغة: | English |
| تدمد: | 1545-7885 1544-9173 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2f51a5668b20ad9bad55c7cb6e86f0c http://europepmc.org/articles/PMC6837583 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d2f51a5668b20ad9bad55c7cb6e86f0c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15457885 15449173 |
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