Quantifying topography-guided actin dynamics across scales using optical flow
| العنوان: | Quantifying topography-guided actin dynamics across scales using optical flow |
|---|---|
| المؤلفون: | Wolfgang Losert, Matt Hourwitz, Phillip Alvarez, Ava Omidvar, Leonard Campanello, Rachel M. Lee, John T. Fourkas |
| المصدر: | Molecular Biology of the Cell |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Cell type, Materials science, Optical flow, HL-60 Cells, macromolecular substances, Biology, Mechanotransduction, Cellular, Models, Biological, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, Actin dynamics, Image Processing, Computer-Assisted, Humans, Nanotopography, Mechanotransduction, Molecular Biology, Cytoskeleton, Actin, Mechanical Phenomena, 030304 developmental biology, 0303 health sciences, Hl60 cells, Epithelial Cells, Articles, Cell Biology, Actin cytoskeleton, Actins, Actin Cytoskeleton, Biophysics, 030217 neurology & neurosurgery, Submicron scale |
| الوصف: | The dynamic rearrangement of the actin cytoskeleton is an essential component of many mechanotransduction and cellular force generation pathways. Here we use periodic surface topographies with feature sizes comparable to those of in vivo collagen fibers to measure and compare actin dynamics for two representative cell types that have markedly different migratory modes and physiological purposes: slowly migrating epithelial MCF10A cells and polarizing, fast-migrating, neutrophil-like HL60 cells. Both cell types exhibit reproducible guidance of actin waves (esotaxis) on these topographies, enabling quantitative comparisons of actin dynamics. We adapt a computer-vision algorithm, optical flow, to measure the directions of actin waves at the submicron scale. Clustering the optical flow into regions that move in similar directions enables micron-scale measurements of actin-wave speed and direction. Although the speed and morphology of actin waves differ between MCF10A and HL60 cells, the underlying actin guidance by nanotopography is similar in both cell types at the micron and submicron scales. |
| تدمد: | 1939-4586 1059-1524 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d38e55a2d4f3eebd2c03ce5b3cfd9249 https://doi.org/10.1091/mbc.e19-11-0614 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d38e55a2d4f3eebd2c03ce5b3cfd9249 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
|---|