Endurance Exercise Ability in the Horse: A Trait with Complex Polygenic Determinism
| العنوان: | Endurance Exercise Ability in the Horse: A Trait with Complex Polygenic Determinism |
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| المؤلفون: | Eric Barrey, Gwendoline Torquet, Caroline Morgenthaler, Laurent Schibler, Céline Robert, Anne Ricard, Núria Mach, Fanny Baste, Christine Blouin, Julie Rivière, Xavier Mata |
| المساهمون: | Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Institut Français du Cheval et de l'Equitation, École nationale vétérinaire d'Alfort (ENVA) |
| المصدر: | Frontiers in Genetics Frontiers in Genetics, Frontiers, 2017, 8, Non paginé. ⟨10.3389/fgene.2017.00089⟩ Frontiers in Genetics (8), Non paginé. (2017) Frontiers in Genetics, Vol 8 (2017) |
| بيانات النشر: | Frontiers Media SA, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:QH426-470, [SDV]Life Sciences [q-bio], Single-nucleotide polymorphism, Genome-wide association study, Quantitative trait locus, Biology, 03 medical and health sciences, Endurance training, Genotype, Genetics, GWAS, Gene, Genetics (clinical), Original Research, genotyping, exercise, endurance, horse, SLC39A12, KCNQ10T1, SORCS3, [SDV.GEN]Life Sciences [q-bio]/Genetics, KCNQ1OT1, 0402 animal and dairy science, Chromosome, 04 agricultural and veterinary sciences, 040201 dairy & animal science, lcsh:Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, 030104 developmental biology, Molecular Medicine |
| الوصف: | International audience; Endurance horses are able to run at more than 20 km/h for 160 km (in bouts of 3040 km). This level of performance is based on intense aerobic metabolism, effective body heat dissipation and the ability to endure painful exercise. The known heritabilities of endurance performance and exercise-related physiological traits in Arabian horses suggest that adaptation to extreme endurance exercise is influenced by genetic factors. The objective of the present genome-wide association study (GWAS) was to identify single nucleotide polymorphisms (SNPs) related to endurance racing performance in 597 Arabian horses. The performance traits studied were the total race distance, average race speed and finishing status (qualified, eliminated or retired). We used three mixed models that included a fixed allele or genotype effect and a random, polygenic effect. Quantile-quantile plots were acceptable, and the regression coefficients for actual vs. expected log(10) p-values ranged from 0.865 to 1.055. The GWAS revealed five significant quantitative trait loci (QTL) corresponding to 6 SNPs on chromosomes 6, 1, 7, 16, and 29 (two SNPs) with corrected p-values from 1.7 x 10(-6) to 1.8 x 10(-5). Annotation of these 5 QTL revealed two genes: sortilin-related VPS10-domain-containing receptor 3 (SORCS3) on chromosome 1 is involved in protein trafficking, and solute carrier family 39 member 12 (SLC39A12) on chromosome 29 is active in zinc transport and cell homeostasis. These two coding genes could be involved in neuronal tissues (CNS). The other QTL on chromosomes 6, 7, and 16 may be involved in the regulation of the gene expression through non-coding RNAs, CpG islands and transcription factor binding sites. On chromosome 6, a new candidate equine long non-coding RNA (KCNQ1OT1 ortholog: opposite antisense transcript 1 of potassium voltage-gated channel subfamily Q-member 1 gene) was predicted in silico and validated by RT-qPCR in primary cultures of equine myoblasts and fibroblasts. This lncRNA could be one element of the cardiac rhythm regulation. Our GWAS revealed that equine performance during endurance races is a complex polygenic trait, and is partially governed by at least 5 QTL: two coding genes involved in neuronal tissues and three other loci with many regulatory functions such as slowing down heart rate. |
| وصف الملف: | application/pdf |
| تدمد: | 1664-8021 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d398fbc948a868e6fb5a4d12c2cf4508 https://doi.org/10.3389/fgene.2017.00089 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d398fbc948a868e6fb5a4d12c2cf4508 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16648021 |
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