Simple, direct drug susceptibility testing technique for diagnosis of drug-resistant tuberculosis in resource-poor settings
| العنوان: | Simple, direct drug susceptibility testing technique for diagnosis of drug-resistant tuberculosis in resource-poor settings |
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| المؤلفون: | Sejoong Kim, Joo Yt, Kim Ck, Lee Ep, Hyun-Jib Kim, Park Yk |
| المصدر: | The International Journal of Tuberculosis and Lung Disease. 17:1212-1216 |
| بيانات النشر: | International Union Against Tuberculosis and Lung Disease, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Veterinary medicine, Tuberculosis, Cost-Benefit Analysis, Antitubercular Agents, Microbial Sensitivity Tests, Mycobacterium tuberculosis, Predictive Value of Tests, Drug Resistance, Multiple, Bacterial, Tuberculosis, Multidrug-Resistant, medicine, Humans, Developing Countries, Tuberculosis, Pulmonary, Resource poor, Dose-Response Relationship, Drug, biology, business.industry, Isoniazid, Sputum, Health Care Costs, Drug susceptibility, Reference Standards, Prognosis, bacterial infections and mycoses, medicine.disease, biology.organism_classification, Infectious Diseases, Calibration, Health Resources, Ofloxacin, medicine.symptom, business, Rifampicin, medicine.drug |
| الوصف: | SETTING The Korean Institute of Tuberculosis, Seoul, Republic of Korea. OBJECTIVE To develop a simple, direct drug susceptibility testing (DST) technique using Kudoh-modified Ogawa (KMO) medium. DESIGN The critical concentrations of isoniazid (INH), rifampicin (RMP), kanamycin (KM) and ofloxacin (OFX) for KMO medium were calibrated by comparing the minimal inhibitory concentrations (MICs) against clinical isolates of Mycobacterium tuberculosis on KMO with those on Lowenstein-Jensen (LJ). The performance of the direct KMO DST technique was evaluated on 186 smear-positive sputum specimens and compared with indirect LJ DST. RESULTS Agreement of MICs on direct vs. indirect DST was high for INH, RMP and OFX. KM MICs on KMO were ∼10 g/ml higher than those on LJ. The critical concentrations of INH, RMP, OFX and KM for KMO were therefore set at 0.2, 40.0, 2.0, and 40.0 g/ml. The evaluation of direct DST of smear-positive sputum specimens showed 100% agreement with indirect LJ DST for INH and RMP. However, the respective susceptible and resistant predictive values were 98.8% and 100% for OFX, and 100% and 80% for KM. CONCLUSION Direct DST using KMO is useful, with clear advantages of a shorter turnaround time, procedural simplicity and low cost compared to indirect DST. It may be most indicated in resource-poor settings for programmatic management of drug-resistant tuberculosis. |
| تدمد: | 1815-7920 1027-3719 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3d1309c8858710372754dd2857054c5 https://doi.org/10.5588/ijtld.12.1009 |
| رقم الأكسشن: | edsair.doi.dedup.....d3d1309c8858710372754dd2857054c5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18157920 10273719 |
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