Decreased Intracellular pH Induced by Cariporide Differentially Contributes to Human Umbilical Cord-Derived Mesenchymal Stem Cells Differentiation
| العنوان: | Decreased Intracellular pH Induced by Cariporide Differentially Contributes to Human Umbilical Cord-Derived Mesenchymal Stem Cells Differentiation |
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| المؤلفون: | Zhenqing Xie, Zhongchao Han, Hairui Zhang, Guoqiang Chang, Qinghua Li, Wei Gao, Tianxiang Pang, Li Ma, Hanyu Wang |
| المصدر: | Cellular Physiology and Biochemistry, Vol 33, Iss 1, Pp 185-194 (2014) |
| بيانات النشر: | S. Karger AG, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Sodium-Hydrogen Exchangers, Physiology, Intracellular pH, Cellular differentiation, Intracellular Space, Intracellular pH (pHi), Biology, Guanidines, lcsh:Physiology, Umbilical Cord, lcsh:Biochemistry, chemistry.chemical_compound, Osteogenesis, Osteogenic differentiation, Humans, lcsh:QD415-436, Sulfones, RNA, Small Interfering, Cation Transport Proteins, beta Catenin, Adipogenesis, Sodium-Hydrogen Exchanger 1, lcsh:QP1-981, Cell Death, Cariporide, Cell growth, Mesenchymal stem cell, Cell Differentiation, Mesenchymal Stem Cells, β-catenin, Hydrogen-Ion Concentration, Up-Regulation, Cell biology, Na+/H+ exchanger 1 (NHE1), Phenotype, Biochemistry, chemistry, Gene Knockdown Techniques, Adipogenic differentiation, Signal transduction, K562 cells |
| الوصف: | Background/Aims: Na+/H+ exchanger 1 (NHE1) is an important regulator of intracellular pH (pHi). High pHi is required for cell proliferation and differentiation. Our previous study has proven that the pHi of mesenchymal stem cells is higher than that of normal differentiated cells and similar to tumor cells. NHE1 is highly expressed in both mesenchymal stem cells and tumor cells. Targeted inhibition of NHE1 could induce differentiation of K562 leukemia cells. In the present paper we explored whether inhibition of NHE1 could induce differentiation of mesenchymal stem cells. Methods: MSCs were obtained from human umbilical cord and both the surface phenotype and functional characteristics were analyzed. Selective NHE1 inhibitor cariporide was used to treat human umbilical cord-derived mesenchymal stem cells (hUC-MSCs). The pHi and the differentiation of hUC-MSCs were compared upon cariporide treatment. The putative signaling pathway involved was also explored. Results: The pHi of hUC-MSCs was decreased upon cariporide treatment. Cariporide up-regulated the osteogenic differentiation of hUC-MSCs while the adipogenic differentiation was not affected. For osteogenic differentiation, β-catenin expression was up-regulated upon cariporide treatment. Conclusion: Decreased pHi induced by cariporide differentially contributes to hUC-MSCs differentiation. |
| تدمد: | 1421-9778 1015-8987 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3da240921275d52f056c6df3472a5d8 https://doi.org/10.1159/000356661 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d3da240921275d52f056c6df3472a5d8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219778 10158987 |
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