Chemical-Empowered Human Adipose-Derived Stem Cells with Lower Immunogenicity and Enhanced Pro-angiogenic Ability Promote Fast Tissue Regeneration
| العنوان: | Chemical-Empowered Human Adipose-Derived Stem Cells with Lower Immunogenicity and Enhanced Pro-angiogenic Ability Promote Fast Tissue Regeneration |
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| المؤلفون: | Junzhi Yi, Jiayan Zhang, Qin Zhang, Xuri Chen, Rujie Qi, Renjie Liang, Ying Wang, Fei Wang, Yuliang Zhong, Xianzhu Zhang, Grace Chin, Qi Liu, Wenyan Zhou, Hua Liu, Jiansong Chen, Hongwei Ouyang |
| المصدر: | Stem Cells Translational Medicine. 11:552-565 |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Wound Healing, Adipose Tissue, Leukocytes, Mononuclear, Endothelial Cells, Humans, Neovascularization, Physiologic, Mesenchymal Stem Cells, Cell Biology, General Medicine, Cells, Cultured, Developmental Biology |
| الوصف: | Mesenchymal stem cells (MSCs) have been widely used as functional components in tissue engineering. However, the immunogenicity and limited pro-angiogenic efficacy of MSCs greatly limited their pro-regenerative ability in allogenic treatment. Herein, utilizing a chemically defined cocktail in the culture system, including cytokines, small molecules, structural protein, and other essential components, we generated the immunoprivileged and pro-angiogenic cells (IACs) derived from human adipose tissues. Conventional adipose-derived MSCs (cADSCs) were used as a control in all the experiments. IACs show typical MSC properties with enhanced stemness capacity and a robust safety profile. IACs induce a significantly milder immune response of allogenic peripheral blood mononuclear cells in an H3K27me3-HLA axis-dependent manner. IACs, through superior paracrine effects, further promote nitric oxide production, anti-apoptotic ability, and the tube formation of human vein endothelial cells. Embedded in a photo-reactive hydrogel (Gel) termed as GelMA/HA-NB/LAP for tissue engineering treatment, IACs promote faster tissue regeneration in a xenogeneic full-thickness skin defect model, eliciting a milder immune response and enhanced blood vessel formation in IACs-treated defect areas. Together with its excellent pro-regenerative potential and robust safety, our findings suggest that IACs may be a promising candidate for clinically relevant stem cell and tissue engineering therapeutics. |
| تدمد: | 2157-6580 2157-6564 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3dace28e58355260afeae2be27c6013 https://doi.org/10.1093/stcltm/szac016 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d3dace28e58355260afeae2be27c6013 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21576580 21576564 |
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