Sphingosine-1-phosphate acts as a developmental stage specific inhibitor of platelet-derived growth factor-induced chemotaxis of osteoblasts
| العنوان: | Sphingosine-1-phosphate acts as a developmental stage specific inhibitor of platelet-derived growth factor-induced chemotaxis of osteoblasts |
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| المؤلفون: | Ilse Steeghs, J. van de Ven, R. Akkers, M. Apotheker, T. Roelofsen, A. Garritsen, Koen J. Dechering, W. Beumer, C. Gelderblom |
| المصدر: | Journal of Cellular Biochemistry, 105, 1128-1138 Journal of Cellular Biochemistry, 105, 4, pp. 1128-1138 |
| بيانات النشر: | Wiley, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | medicine.medical_specialty, Applied Biology, Bone morphogenetic protein 8A, Biology, Bone morphogenetic protein, Biochemistry, Bone morphogenetic protein 2, Bone remodeling, Mice, Osteogenesis, Sphingosine, Internal medicine, medicine, Animals, Humans, Molecular Biology, Cells, Cultured, Osteoblasts, Chemotaxis, Cell Differentiation, Osteoblast, Proto-Oncogene Proteins c-sis, Cell Biology, Cell biology, Bone morphogenetic protein 7, Receptors, Lysosphingolipid, Bone morphogenetic protein 6, Bone morphogenetic protein 5, medicine.anatomical_structure, Endocrinology, Lysophospholipids |
| الوصف: | The development and maintenance of a healthy skeleton depends on the migration of cells to areas of new bone formation. Osteoblasts, the bone forming cells of the body, mature from mesenchymal stem cells under the influence of bone morphogenetic protein. It is unclear at what developmental stage the osteoblasts start to migrate to their functional location. We have studied migration of immature pre-osteoblasts and of mature osteoblasts in response to Platelet-derived growth factor (PDGF) and sphingosine-1-phosphate (S1P). PDGF is a growth factor involved in bone remodeling and fracture healing whereas S1P is a circulating sphingolipid known to control cell trafficking. Our data indicate that PDGF acts as a chemotactic cue for pre-osteoblasts. In contrast, S1P is a chemorepellent to these cells. Upon Bone Morphogenetic Protein 2-induced conversion to the osteoblast phenotype, the chemotaxis response to PDGF is retained whereas the sensitivity to S1P is lost. By RNA interference and overexpression experiments we showed that the expression level of the S1P2 receptor is the sole determinant controlling responsiveness to S1P. The combined data indicate that migration of osteoblasts is controlled by the balance between PDGF, S1P and the differentiation state of the cells. We propose that this mechanism preserves the osteoprogenitor pool in the bone marrow, only allowing the more differentiated cell to travel to sites of bone formation. |
| وصف الملف: | application/pdf |
| تدمد: | 1097-4644 0730-2312 1128-1138 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4605af17d1c005b2314545b524ec8bf https://doi.org/10.1002/jcb.21915 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d4605af17d1c005b2314545b524ec8bf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974644 07302312 11281138 |
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