Effects of Axial Compression and Distraction on Vascular Bud and VEGFA Expression in the Vertebral Endplate of an Ex Vivo Rabbit Spinal Motion Segment Culture Model
| العنوان: | Effects of Axial Compression and Distraction on Vascular Bud and VEGFA Expression in the Vertebral Endplate of an Ex Vivo Rabbit Spinal Motion Segment Culture Model |
|---|---|
| المؤلفون: | Ping Zhang, Min-Shan Feng, Rui Xie, Kai Sun, Chen Ming, Liguo Zhu, Xunlu Yin, Xu Wei, He Yin, Zhan Jiawen, Shang-Quan Wang, Jing-Hua Gao, Jie Yu |
| المصدر: | Spine. 46:421-432 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Vascular Endothelial Growth Factor A, Intervertebral Disc Degeneration, Weight-Bearing, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Downregulation and upregulation, Pressure, Animals, Medicine, Orthopedics and Sports Medicine, Range of Motion, Articular, Intervertebral Disc, Receptor, Aggrecan, 030222 orthopedics, Lumbar Vertebrae, business.industry, Kinase insert domain receptor, Intervertebral disc, Anatomy, Vascular endothelial growth factor, Vascular endothelial growth factor A, medicine.anatomical_structure, chemistry, Rabbits, Neurology (clinical), business, Spinal Cord Compression, Intervertebral Disc Displacement, 030217 neurology & neurosurgery, Ex vivo |
| الوصف: | STUDY DESIGN An ex vivo study of the rabbit's vertebral endplate. OBJECTIVE The aim of this study was to assess the effect of axial compression and distraction on vascular buds and vascular endothelial growth factor (VEGFA) expression of the vertebral endplate (VEP). SUMMARY OF BACKGROUND DATA The abnormal load can lead to intervertebral disc degeneration (IDD), whereas axial distraction can delay this process. The effects of different mechanical loads on the intervertebral disc (IVD) have been hypothesized to be related to changes in the vascular buds of the VEP; moreover, the process that might involve the vascular endothelial growth factor (VEGF) within the VEP. METHODS Rabbit spinal segments (n = 40) were harvested and randomly classified into four groups: Control group, no stress was applied; Group A, a constant compressive load applied; Group B, compression load removed for a fixed time daily on a continuous basis, and substituted with a distraction load for 30 minutes; and Group C, compression removed for 30 minutes for a fixed period daily on a continuous basis. Tissue specimens were collected before the culture (day 0) and on day 14 post-culture of each group for analysis of IVDs' morphology, and protein and mRNA expression of Aggrecan, COL2al, VEGFA, and vascular endothelial growth factor receptor 2 of the VEPs. RESULTS Application of axial distraction and dynamic load compression significantly delayed time- and constant compression-mediated VEP changes and IDD. Moreover, the degree of degeneration was associated with loss of vascular buds, as well as the downregulation of VEGFA and its receptor. CONCLUSION The regulation of vascular buds and VEGF expression in the VEP represents one of the mechanisms of axial distraction and dynamic loading.Level of Evidence: N/A. |
| تدمد: | 1528-1159 0362-2436 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d49d0f7214fe35bcba0d982b1a2d7db7 https://doi.org/10.1097/brs.0000000000003816 |
| رقم الأكسشن: | edsair.doi.dedup.....d49d0f7214fe35bcba0d982b1a2d7db7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15281159 03622436 |
|---|