التفاصيل البيبلوغرافية
| العنوان: |
An integrated 5-Mb physical, genetic, and radiation hybrid map of a 1p36.1 region implicated in neuroblastoma pathogenesis |
| المؤلفون: |
Nicole Spieker, Frank Speleman, Huib N. Caron, Ilja Roobeek, Johan T. den Dunnen, Mabel Beitsma, Rogier Versteeg, Peter van Sluis |
| المساهمون: |
Other departments |
| المصدر: |
Genes, chromosomes & cancer, 27(2), 143-152. Wiley-Liss Inc. |
| سنة النشر: |
2000 |
| مصطلحات موضوعية: |
Genetic Markers, Cancer Research, Genetic Vectors, Molecular Sequence Data, Genes, myc, Locus (genetics), Hybrid Cells, Biology, law.invention, Contig Mapping, Neuroblastoma, law, Tumor Cells, Cultured, Genetics, medicine, Humans, Radiation hybrid mapping, Bacteriophage P1, Gene, neoplasms, Expressed Sequence Tags, Breakpoint, Gene Amplification, Chromosome, Physical Chromosome Mapping, medicine.disease, Molecular biology, Electrophoresis, Gel, Pulsed-Field, Telomere, Genes, Chromosomes, Human, Pair 1, Suppressor |
| الوصف: |
Common genetic aberrations of neuroblastoma are deletions of the short arm of chromosome 1 (1p36) and MYCN amplification. Our deletion analysis of 25 tumor cell lines and 171 tumors strongly suggests that 1p harbors several tumor suppressor loci. Distinct loci are involved in MYCN single-copy versus MYCN-amplified neuroblastoma. Deletions in MYCN single-copy tumors have a shortest region of overlap (SRO) of 20 cM at 1p36.3. MYCN-amplified tumors have large deletions with an SRO of about 60 cM, from 1p36.1 to the telomere. This SRO is defined by D1S7 (1p36.1), which was the most distal locus retained. Therefore, a suppressor gene associated with MYCN-amplified tumors probably maps within a few megabases distal of D1S7. In order to map this locus, we further refined this SRO. We mapped the breakpoint of the MYCN-amplified neuroblastoma with the smallest 1p deletion between 56.6 and 57.2 cM from 1pter. Pulsed-field gel electrophoresis and radiation hybrid mapping were used to construct a 5-Mb physical map of this region. The map includes the region from 82.73 till 92.89 cR from 1pter. About half of it was isolated in P1 and PAC clones. The region harbors the genes FGR, SLC9A1, HMG17, EXTL1, AML2, RH, OP18, four ESTs, and a newly identified gene with a transcript size of approximately 7 Kb. Several of the mapped genes have a putative role in cell growth, differentiation, and morphogenesis. Genes Chromosomes Cancer 27:143–152, 2000. © 2000 Wiley-Liss, Inc. |
| اللغة: |
English |
| تدمد: |
1045-2257 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4b172d6a2679b695054e7969e45ed41
https://doi.org/10.1002/(sici)1098-2264(200002)27:2<143::aid-gcc5>3.0.co;2-e |
| حقوق: |
RESTRICTED |
| رقم الأكسشن: |
edsair.doi.dedup.....d4b172d6a2679b695054e7969e45ed41 |
| قاعدة البيانات: |
OpenAIRE |
