Effects of 1,2-Dimethylhydrazine on Barrier Properties of Rat Large Intestine and IPEC-J2 Cells
| العنوان: | Effects of 1,2-Dimethylhydrazine on Barrier Properties of Rat Large Intestine and IPEC-J2 Cells |
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| المؤلفون: | Linda Droessler, Viktoria V. Bekusova, Alexander G. Markov, Salah Amasheh |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 10278, p 10278 (2021) International Journal of Molecular Sciences Volume 22 Issue 19 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 1,2-dimethylhydrazine, Swine, Colorectal cancer, Cell, barrier properties, medicine.disease_cause, chemistry.chemical_compound, Electric Impedance, Intestinal Mucosa, Biology (General), Spectroscopy, Tight junction, General Medicine, Computer Science Applications, 1,2-Dimethylhydrazine, Chemistry, medicine.anatomical_structure, Paracellular transport, Colonic Neoplasms, tight junction proteins, Immunohistochemistry, endocrine system, QH301-705.5, colorectal cancer, Adenocarcinoma, 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten, Article, Permeability, Catalysis, Cell Line, Inorganic Chemistry, medicine, Animals, Rats, Wistar, Physical and Theoretical Chemistry, Molecular Biology, QD1-999, Organic Chemistry, medicine.disease, Molecular biology, Rats, IPEC-J2, chemistry, Permeability (electromagnetism), Claudins, Carcinogens, transepithelial electrical resistance, Carcinogenesis |
| الوصف: | Colon cancer is accompanied by a decrease of epithelial barrier properties, which are determined by tight junction (TJ) proteins between adjacent epithelial cells. The aim of the current study was to analyze the expression of TJ proteins in a rat model of 1,2-dimethylhydrazine (DMH)-induced colorectal cancer, as well as the barrier properties and TJ protein expression of IPEC-J2 cell monolayers after incubation with DMH. Transepithelial electrical resistance and paracellular permeability for sodium fluorescein of IPEC-J2 were examined by an epithelial volt/ohm meter and spectrophotometry. The expression and localization of TJ proteins were analyzed by immunoblotting and immunohistochemistry. In the colonic tumors of rats with DMH-induced carcinogenesis, the expression of claudin-3 and -4 was significantly increased compared to controls. The transepithelial electrical resistance of IPEC-J2 cells increased, while paracellular permeability for sodium fluorescein decreased, accompanied by an increased expression of claudin-4. The increase of claudin-4 in rat colon after chronic DMH exposure was consistent with the acute effect of DMH on IPEC-J2 cells, which may indicate an essential role of this protein in colorectal cancer development. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4bfcf02dc62c11d1e0d31b425738d75 https://www.mdpi.com/1422-0067/22/19/10278 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d4bfcf02dc62c11d1e0d31b425738d75 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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