RET/PTC1 oncogene signaling in PC Cl 3 thyroid cells requires the small GTP-binding protein Rho
| العنوان: | RET/PTC1 oncogene signaling in PC Cl 3 thyroid cells requires the small GTP-binding protein Rho |
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| المؤلفون: | Rosa Marina Melillo, Giovanni Santelli, A. Mineo, Leandra Sepe, Massimo Santoro, Carmen Monaco, Alfredo Fusco, Donatella Tramontano, Maria Vittoria Barone, Maria Domenica Castellone |
| المساهمون: | Barone, MARIA VITTORIA, Sepe, L, Melillo, Rm, Mineo, A, Santelli, G, Monaco, C, Castellone, Md, Tramontano, Donatella, Fusco, A, Santoro, M., Melillo, ROSA MARINA, Fusco, Alfredo, Santoro, Massimo |
| المصدر: | Oncogene (Basingstoke) 20 (2001): 6973–6982. info:cnr-pdr/source/autori:Barone M.V. 1, Sepe L. 2, Melillo R.M. 1, Mineo A. 2, Santelli G. 2, Monaco C. 1, Castellone M.D. 1, Tramontano D. 3, Fusco A. 1, Santoro M. 1/titolo:RET%2FPTC1 oncogene signaling in PC Cl 3 thyroid cells requires the small GTP-binding protein Rho./doi:/rivista:Oncogene (Basingstoke)/anno:2001/pagina_da:6973/pagina_a:6982/intervallo_pagine:6973–6982/volume:20 |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | rho GTP-Binding Proteins, Cancer Research, endocrine system diseases, Oncogene Proteins, Fusion, Thyroid Gland, Apoptosis, Multiple Endocrine Neoplasia Type 2a, tyrosine-kinase, thyroid, Mice, Stress Fibers, Tumor Cells, Cultured, Drosophila Proteins, Cell Line, Transformed, Kinase, 3T3 Cells, Protein-Tyrosine Kinases, Neoplasm Proteins, Phenotype, Organ Specificity, Female, actin, Tyrosine kinase, Dimerization, Proto-oncogene tyrosine-protein kinase Src, Signal Transduction, DNA Replication, congenital, hereditary, and neonatal diseases and abnormalities, endocrine system, Stress fiber, Cell Survival, MAP Kinase Signaling System, Recombinant Fusion Proteins, Breast Neoplasms, Biology, Adenocarcinoma, Transfection, RET Oncoprotein, Cell Line, Thyroid carcinoma, Rho, Proto-Oncogene Proteins, Genetics, Animals, Humans, Neoplasm Invasiveness, Thyroid Neoplasms, Ra, Molecular Biology, neoplasms, Thyroid Epithelial Cells, Oncogene, Proto-Oncogene Proteins c-ret, Membrane Proteins, Receptor Protein-Tyrosine Kinases, apoptosi, Actins, Carcinoma, Papillary, Rac, Rats, Cancer research, RET |
| الوصف: | Thyroid papillary carcinomas are characterized by RET/PTC rearrangements that cause the tyrosine kinase domain of the RET receptor to fuse with N-terminal sequences encoded by heterologous genes. This results in the aberrant expression of a ligand-independent and constitutively active RET kinase. We analysed actin reorganization induced by the RET/PTC1 oncogene in PC Cl 3 rat thyroid epithelial cells. Differently from oncogenes Src, Ras and Raf, RET/PTC1 caused actin filaments to form prominent stress fibers. Moreover, stress fibers were identified in human thyroid papillary carcinoma cell lines harboring RET/PTC1 rearrangements but not in thyroid carcinoma cells negative for RET/PTC rearrangements. RET/MEN 2A, a constitutively active but unrearranged membrane-bound RET oncoprotein, did not induce stress fibers in PC Cl 3 cells. Induction of stress fibers by RET/PTC1 was restricted to thyroid cells; it did not occur in NIH3T3 fibroblasts or MCF7 mammary cells. RET/PTC1-mediated stress fiber formation depended on Rho but not Rac small GTPase activity. In addition, inhibition of Rho, but not of Rac, caused apoptosis of RET/PTC1-expressing thyroid cells. We conclude that Rho is implicated in the actin reorganization and cell survival mediated by the chimeric RET/PTC1 oncogene in thyroid epithelial cells, both phenotypes being cell type- and oncogene type-specific. |
| وصف الملف: | STAMPA |
| تدمد: | 0950-9232 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4dbeb4ed7aac36f7db5270d2589e776 https://pubmed.ncbi.nlm.nih.gov/11704822 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d4dbeb4ed7aac36f7db5270d2589e776 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09509232 |
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