DNA barcoding and molecular phylogeny of Dumasia (Fabaceae: Phaseoleae) reveals a cryptic lineage
| العنوان: | DNA barcoding and molecular phylogeny of Dumasia (Fabaceae: Phaseoleae) reveals a cryptic lineage |
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| المؤلفون: | Bin Tian, Kai-Wen Jiang, Zhong-Fu Zhang, Bo Pan, Rong Zhang |
| المصدر: | Plant Diversity Plant Diversity, Vol 42, Iss 5, Pp 376-385 (2020) |
| بيانات النشر: | Kunming Institute of Botany, Chinese Academy of Sciences, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, Species complex, Plant Science, Biology, 010603 evolutionary biology, 01 natural sciences, DNA barcoding, Monophyly, lcsh:Botany, Phaseoleae, lcsh:QH301-705.5, Ecology, Evolution, Behavior and Systematics, Phylogenetic tree, Internal transcribed spacer (ITS), Fabaceae, biology.organism_classification, lcsh:QK1-989, Plastid genome, Chloroplast DNA, lcsh:Biology (General), Dumasia, Evolutionary biology, Molecular phylogenetics, Cryptic species, 010606 plant biology & botany, Research Paper |
| الوصف: | Dumasia taxonomy and classification have long been problematic. Species within this genus have few morphological differences and plants without flowers or fruits are difficult to accurately identify. In this study, we evaluated the ability of six DNA barcoding sequences, one nuclear (ITS) and five chloroplast regions (trnH-psbA, matK, rbcL, trnL-trnF, psbB-psbF), to efficiently identify Dumasia species. Most single markers or their combinations identify obvious barcoding gaps between intraspecific and interspecific genetic variation. Most combined analyses including ITS showed good species resolution and identification efficiency. We therefore suggest that ITS alone or a combination of ITS with any cpDNA marker are most suitable for DNA barcoding of Dumasia. The phylogenetic analyses clearly indicated that Dumasia yunnanensis is not monophyletic and is separated as two independent branches, which may result from cryptic differentiation. Our results demonstrate that molecular data can deepen the comprehension of taxonomy of Dumasia and provide an efficient approach for identification of the species. |
| اللغة: | English |
| تدمد: | 2468-2659 2096-2703 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d56870a1480aa6bbdb60309ffd1cf1de http://europepmc.org/articles/PMC7584798 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d56870a1480aa6bbdb60309ffd1cf1de |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24682659 20962703 |
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