Mechano‐regulation of Wnt/ β‐Catenin signaling to control paraxial versus lateral mesoderm lineage bifurcation
| العنوان: | Mechano‐regulation of Wnt/ |
|---|---|
| المؤلفون: | Tobias Strittmatter, Viktor Haellman, Paul Argast, Peter Buchman, Ana Palma Teixeira, Martin Fussenegger |
| المصدر: | Biotechnology Progress. 39 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Biotechnology |
| الوصف: | Mechanical cues are involved in many biological processes, including embryonic development and patterning. For example, external mechanical forces (shear stress), lateral cell-cell interactions, and mechanical properties (stiffness and composition) of the extracellular matrix are thought to modulate Wnt signaling, which is a highly conserved pathway involved in regulating stem cell renewal, proliferation, and differentiation. In this work, we employed a customized higher-throughput shear stress induction device for the controlled application of mechanical stress to study the effects of shear stress on the differentiation of human induced pluripotent stem cells (hiPSCs) toward the three germ layers. We found that mechanical stress alters lineage commitment during ectoderm and mesoderm differentiation. We show that this effect correlates with reduced Wnt signaling, evaluated in terms of the promoter activity of an established TCF3-responsive promoter. Whole transcriptome sequencing and pathway enrichment analysis of the differentially expressed genes between hiPSC-derived mesoderm cells differentiated in the presence or absence of piston-induced shear stress confirmed that Wnt/β-catenin signaling is among the most affected developmental pathways. Furthermore, our results suggest that suitably programmed shear stress application could be used to selectively promote differentiation of hiPSCs to either lateral or paraxial mesoderm in commercially available media. |
| تدمد: | 1520-6033 8756-7938 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d57475e8a1d4cf59b37ad573b250a817 https://doi.org/10.1002/btpr.3312 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d57475e8a1d4cf59b37ad573b250a817 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15206033 87567938 |
|---|