Mathematical model of polymerase chain reaction with temperature-dependent parameters
| العنوان: | Mathematical model of polymerase chain reaction with temperature-dependent parameters |
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| المؤلفون: | M V Dubina, V. E. Kurochkin, D. G. Sochivko, Y I Alekseev, Alexey Fedorov |
| المصدر: | Doklady Biochemistry and Biophysics. 472:77-80 |
| بيانات النشر: | Pleiades Publishing Ltd, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Base pair, Stereochemistry, Kinetics, Biophysics, Thermodynamics, Nucleic Acid Denaturation, Polymerase Chain Reaction, 01 natural sciences, Biochemistry, Dissociation (chemistry), law.invention, chemistry.chemical_compound, Reaction rate constant, law, 0103 physical sciences, Taq Polymerase, Base Pairing, Polymerase chain reaction, 010302 applied physics, 010304 chemical physics, Temperature, DNA, General Chemistry, General Medicine, Models, Theoretical, chemistry, Reagent |
| الوصف: | The course of the real-time polymerase chain reaction (PCR) is determined by the temperature dependence of the kinetics of the component reactions, particularly the DNA strand hybridization. To investigate the effect of thermal processes on the reaction behavior, a mathematical model in which the variable rate constant of dissociation of "primer-single strand" complexes depends on temperature was proposed. The reaction medium temperature, which depends on time, was also introduced into the model. The proposed model of real-time PCR makes it possible to analyze different aspects of the reaction, which are important for the development of instruments and reagents for PCR. |
| تدمد: | 1608-3091 1607-6729 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5df63424bb4220534f48c79d68b0163 https://doi.org/10.1134/s1607672917010240 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....d5df63424bb4220534f48c79d68b0163 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16083091 16076729 |
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