Application of Novel 3,4-Dihydroxyphenylalanine-Containing Antimicrobial Polymers for the Prevention of Uropathogen Attachment to Urinary Biomaterials
| العنوان: | Application of Novel 3,4-Dihydroxyphenylalanine-Containing Antimicrobial Polymers for the Prevention of Uropathogen Attachment to Urinary Biomaterials |
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| المؤلفون: | Chris Wattengel, Justin Koepsel, Liam Brennan, Peter A. Cadieux, Jeremy P. Burton, Jeffrey L. Dalsin, Roderick A. MacPhee, Hassan Razvi, Sai K Vangala, Thomas Tailly |
| المصدر: | J Endourol |
| بيانات النشر: | Mary Ann Liebert Inc, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Polymers, Urology, Urinary system, Dopamine Agents, Polyurethanes, 030232 urology & nephrology, In Vitro Techniques, Urinary Catheters, Gram-Positive Bacteria, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Anti-Infective Agents, Coated Materials, Biocompatible, Gram-Negative Bacteria, Materials Testing, Enterococcus faecalis, Escherichia coli, Staphylococcus epidermidis, Humans, Medicine, Experimental Endourology, Proteus mirabilis, Urinary catheter, business.industry, Biofilm, Drug-Eluting Stents, Antimicrobial, Dihydroxyphenylalanine, Anti-Bacterial Agents, Klebsiella pneumoniae, chemistry, Biofilms, Catheter-Related Infections, Staphylococcus saprophyticus, 030220 oncology & carcinogenesis, Pseudomonas aeruginosa, Urinary Tract Infections, Silver Nitrate, business |
| الوصف: | Introduction and Objective: Urinary catheters and stents are frequently prone to catheter-associated urinary tract infections (CAUTI) through biofilm formation. Several strategies have been evaluated in search of a stent coating to reliably prevent adherence of bacteria and biofilm. Previous in vivo and in vitro research with methoxylated polyethylene glycol 3,4-dihydroxyphenylalanine (DOPA) copolymer as a candidate coating showed promising results to reduce the bacterial attachment. We aimed to further enhance this antimicrobial activity by adding antimicrobial agents to newly synthesized DOPA-based copolymers. Materials and Methods: Building on our previous experience, novel copolymers were engineered based on DOPA. Quaternary ammonium groups and silver particles were added by cross-linking to increase the antimicrobial activity through both kill-by-contact and planktonic killing. After coating polyurethane sheets and measuring contact angles, all candidate coatings were challenged in vitro with an Escherichia coli culture. The most promising coatings were then further evaluated against a panel of seven clinically relevant uropathogens and planktonic killing, and microbial attachment was determined. Results: Initially, seven coatings were developed, referred to as Surphys 093–099. The most significant increase in contact angle was identified in Surphys-095 and -098. Surphys coatings S-094, S-095, and S-098 were cross-linked with silver and exhibited profound antimicrobial properties when challenged with E. coli. Further testing demonstrated S-095 to have antimicrobial efficacy against gram-positive and gram-negative bacteria at different silver-loading concentrations. The final coating, consisting of a 2 mg/mL solution of S-095 cross-linked with 0.25 mg/mL AgNO(3), appeared to be highly bactericidal showing a ≥99.9% bacterial killing effect while remaining below cytotoxicity levels. Conclusions: We were able to engineer DOPA-based copolymers and add quaternary ammonium and silver particles, thus increasing the bactericidal properties of the coating. These coatings have exhibited a biologically significant ability to prevent uropathogens from attaching to biomaterials and represent a realistic opportunity to combat CAUTI. |
| تدمد: | 1557-900X 0892-7790 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6292e184fb7d2a8e3eec816ea5f7b19 https://doi.org/10.1089/end.2019.0009 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d6292e184fb7d2a8e3eec816ea5f7b19 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1557900X 08927790 |
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