miR-33 and RIP140 participate in LPS-induced acute lung injury
| العنوان: | miR-33 and RIP140 participate in LPS-induced acute lung injury |
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| المؤلفون: | Wansi Zhong, Huan Hou, Nianlong Yan, Hua Li, Shuang Liu, Xiaojuan Hu, Yangyang Feng |
| المصدر: | Turkish Journal of Medical Sciences Volume: 49, Issue: 1 422-428 |
| بيانات النشر: | The Scientific and Technological Research Council of Turkey, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | miR-33, Lipopolysaccharides, miR-33 and RIP140, Lipopolysaccharide, Acute Lung Injury, H&E stain, Lipopolysaccharide,PMVEC,acute lung injury,miR-33 and RIP140, 030204 cardiovascular system & hematology, Lung injury, Pharmacology, Article, 03 medical and health sciences, chemistry.chemical_compound, Mice, 0302 clinical medicine, Pyrrolidine dithiocarbamate, Western blot, medicine, PMVEC, Animals, Lung, Cells, Cultured, 0303 health sciences, Mice, Inbred BALB C, medicine.diagnostic_test, 030306 microbiology, business.industry, Endothelial Cells, General Medicine, Nuclear Receptor Interacting Protein 1, MicroRNAs, medicine.anatomical_structure, Real-time polymerase chain reaction, chemistry, lipids (amino acids, peptides, and proteins), business |
| الوصف: | Background/aim: Pulmonary microvascular endothelial cells (PMVECs) play a pivotal role in the process of acute lung injury (ALI), which can be induced by lipopolysaccharide (LPS). Numerous reports have indicated that both miR-33 and RIP140 are involved in the inflammatory response in macrophages. In this study, we sought to investigate whether miR-33 and RIP140 participate in ALI induced by LPS.Materials and methods: First, we isolated and identified PMVECs from BALB/c mice. Subsequently, both PMVECs and BALB/c mice were treated with PBS, LPS, or pyrrolidine dithiocarbamate (PDTC) plus LPS and divided into three groups: control (PBS), LPS (LPS), and L+P (LPS plus PDTC) groups. We assessed pathology by hematoxylin and eosin staining, and miR-33 and RIP140 expression levels were examined using quantitative PCR and Western blot analyses.Results: Our results demonstrated that LPS can induce PMVEC injury and ALI and that LPS treatment significantly decreased miR-33 expression compared with controls (P < 0.001, n = 5). On the contrary, RIP140 was markedly overexpressed by LPS treatment (P < 0.001, n = 5). However, this alteration can be inhibited by pretreatment with PDTC before LPS (P < 0.05, n = 5).Conclusion: This study is the first to confirm that both miR-33 and RIP140 participate in LPS-induced PMVEC injury and ALI, which may help uncover the mechanism of ALI. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1303-6165 1300-0144 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d695828b4b334dab3eda997ba44f5066 http://europepmc.org/articles/PMC7350843 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d695828b4b334dab3eda997ba44f5066 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13036165 13000144 |
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