Structure elucidation of the O-antigenic polysaccharide from the enteroaggregative Escherichia coli strain 62D1
| العنوان: | Structure elucidation of the O-antigenic polysaccharide from the enteroaggregative Escherichia coli strain 62D1 |
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| المؤلفون: | Mikael Staaf, Andrej Weintraub, Felipe Urbina, Göran Widmalm |
| المصدر: | European Journal of Biochemistry. 262:56-62 |
| بيانات النشر: | Wiley, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Magnetic Resonance Spectroscopy, Strain (chemistry), Chemistry, Molecular Sequence Data, O Antigens, Carbon-13 NMR, medicine.disease_cause, Biochemistry, NMR spectra database, Crystallography, Carbohydrate Sequence, Heteronuclear molecule, Enteroaggregative Escherichia coli, Carbohydrate Conformation, Escherichia coli, medicine, Proton NMR, Two-dimensional nuclear magnetic resonance spectroscopy |
| الوصف: | The O-antigen polysaccharide of the lipopolysaccharide from the enteroaggregative Escherichia coli strain 62D1 has been determined. Sugar and methylation analysis together with 1H and 13C NMR spectroscopy revealed the components of the repeating unit. Two-dimensional NOESY and heteronuclear multiple-bond correlation experiments were used to deduce the sequence. 1H and 13C NMR spectra indicate heterogeneity in the polysaccharide. Methylation analysis and 1H NMR spectra of native and Smith-degraded material show that the majority (65%) of the repeating units has the following structure: Minor resonances in the NMR spectra are consistent with the presence of repeating units which lack the α-d-Galp terminal residue (35%). |
| تدمد: | 1432-1033 0014-2956 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d71a5b973bb7681a1006adaa3b3e43df https://doi.org/10.1046/j.1432-1327.1999.00319.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d71a5b973bb7681a1006adaa3b3e43df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321033 00142956 |
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