CD73 expression is critical to therapeutic effects of human endometrial regenerative cells in inhibition of cardiac allograft rejection in mice
| العنوان: | CD73 expression is critical to therapeutic effects of human endometrial regenerative cells in inhibition of cardiac allograft rejection in mice |
|---|---|
| المؤلفون: | Hao Wang, Hongda Wang, Yafei Qin, Wang Jin, Dejun Kong, Yiming Zhao, Yonghao Hu, Dingding Yu, Xiang Li, Jingpeng Hao, Guangming Li, Baoren Zhang, Zhaoyan Pang |
| المصدر: | Stem Cells Translational Medicine, Vol 10, Iss 3, Pp 465-478 (2021) Stem Cells Translational Medicine |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Graft Rejection, Pluripotent Stem Cells, 0301 basic medicine, allograft protection, medicine.drug_class, medicine.medical_treatment, cardiac transplantation, Monoclonal antibody, Immune tolerance, Endometrium, Mice, 03 medical and health sciences, 0302 clinical medicine, Animals, Humans, Medicine, lcsh:QH573-671, Receptor, 5'-Nucleotidase, Mice, Inbred BALB C, lcsh:R5-920, business.industry, human endometrial regenerative cells, lcsh:Cytology, Graft Survival, Cell Biology, General Medicine, Allografts, Adenosine receptor, Adenosine, Mice, Inbred C57BL, Transplantation, 030104 developmental biology, Cytokine, adenosine, Cancer research, CD73, Cytokines, Heart Transplantation, Female, business, lcsh:Medicine (General), 030217 neurology & neurosurgery, CD8, Developmental Biology, medicine.drug |
| الوصف: | The newly found mesenchymal‐like endometrial regenerative cells (ERCs) have been proved to induce immune tolerance in cardiac allograft transplantation. However, the therapeutic mechanism is not clear. The present study was undertaken to investigate whether ecto‐5′‐nucleotidase (CD73) expression on ERCs is critical to cardiac allograft protection. C57BL/6 mouse recipients receiving BALB/c mouse cardiac allografts were treated with unmodified ERCs or anti‐CD73 monoclonal antibodies (mAb) pretreated ERCs, respectively. It has been found that CD73 expression was critical to ERC‐induced attenuation of graft pathology. The blockage of CD73 expression on ERCs was related to the percentage decline of tolerogenic dendritic cells (Tol‐DCs), macrophages type 2 (M2), and regulatory T cells (Tregs). As compared with anti‐CD73 mAb pretreated ERCs group, CD73 expressing ERCs significantly increased the level of anti‐inflammatory cytokine IL‐10 but decreased levels of pro‐inflammatory cytokines including IFN‐γ and TNF‐α. In addition, CD73 expressing ERCs showed tissue protective function via the regulation of adenosine receptor expression which was related to the infiltration of CD4+ and CD8+ cells in the allografts. Furthermore, significant increase of A2B receptors in the cardiac allograft was also associated with CD73 expressing ERC‐induced prolongation of cardiac allograft survival. Ecto‐5′‐nucleotidase (CD73) expression is critical to therapeutic effects of human endometrial regenerative cells in inhibition of cardiac allograft rejection in mice. The CD73 expression on human endometrial regenerative cells mainly affects three aspects: immune cells, cytokines, and the tissue expression of adenosine receptors. |
| اللغة: | English |
| تدمد: | 2157-6564 2157-6580 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d76bdad635f9a539ae82f51d1b6b5442 https://doaj.org/article/ba6e07ebabee413290e16cd8e3146d01 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....d76bdad635f9a539ae82f51d1b6b5442 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21576564 21576580 |
|---|