Comparison of the Rhizosphere Soil Microbial Community Structure and Diversity Between Powdery Mildew-Infected and Noninfected Strawberry Plants in a Greenhouse by High-Throughput Sequencing Technology
| العنوان: | Comparison of the Rhizosphere Soil Microbial Community Structure and Diversity Between Powdery Mildew-Infected and Noninfected Strawberry Plants in a Greenhouse by High-Throughput Sequencing Technology |
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| المؤلفون: | Shen Xuemei, Jiangyun Tong, Daifa Su, Xiao-Long Cui, Wei Shijie, Luo Zhiwei, Junyu Yang, Arslan Jamil, Zhengrong Zhang, Chen Shanyan, Yonghong Lai |
| المصدر: | Current Microbiology. 77:1724-1736 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Fragaria, Plant Roots, Applied Microbiology and Biotechnology, Microbiology, Actinobacteria, 03 medical and health sciences, RNA, Ribosomal, 16S, Botany, Soil Microbiology, Plant Diseases, 030304 developmental biology, 0303 health sciences, Rhizosphere, Bacteria, biology, 030306 microbiology, Microbiota, High-Throughput Nucleotide Sequencing, food and beverages, Bacteroidetes, Biodiversity, General Medicine, biology.organism_classification, Archaea, Trichoderma, Proteobacteria, Soil microbiology, Powdery mildew, Acidobacteria |
| الوصف: | The aim of this study was to compare the microbial community structure and diversity in powdery mildew-infected and noninfected strawberry plant rhizosphere soils in the greenhouse based on variations in the 16S rRNA gene V3-V4 and fungal ITS2 regions by Illumina amplicon sequencing. Powdery mildew infection reduced the number of operational taxonomic units (OTUs) and prokaryotic and fungal community richness/diversity indexes in the rhizosphere soils compared with those in healthy plant soils. Furthermore, 3543 prokaryotic and 581 fungal OTUs were obtained at the 97% similarity level. Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, and Chloroflexi were the dominant bacterial phyla; Woesearchaeota_DHVEG-6, Bathyarchaeota, and Thaumarchaeota were the dominant archaea; and Ascomycota, Basidiomycota, unclassified_fungi, and Zygomycota were the dominant fungal phyla. Their proportions differed significantly among samples. Wolbachia, Devosia, Pseudolabrys, Streptomyces, and Rhizomicrobium were the most abundant bacterial genera; their proportions differed significantly among samples. Most Pseudomonas, Streptomyces, and 'norank' group members might be potential antagonistic microorganisms of powdery mildew pathogens, and Wolbachia and Rickettsia might be pathogen-transmitting vectors. Microascus, Clitopilus, and Ciliophora were the dominant fungi, and their community structures and abundances significantly differed among samples. Microascus, Talaromyces, Zopfiella, and Cryptococcus were relatively more abundant in the powdery mildew-infected strawberry plant rhizosphere soils. Fusarium, Trichoderma, Clitopilus, and 'unclassified' group members may be potential antagonistic populations. The results suggested that powdery mildew-infected strawberry fruits and plants cannot be consumed. This report is the first study to illustrate differences in the rhizosphere soil prokaryotic and fungal communities between powdery mildew-infected and noninfected strawberry plants in a greenhouse. |
| تدمد: | 1432-0991 0343-8651 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7700c444d4540caea5c76a935aa68c5 https://doi.org/10.1007/s00284-020-01948-x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....d7700c444d4540caea5c76a935aa68c5 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14320991 03438651 |
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